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目的:建立通脉活血颗粒的定性定量方法。方法:采用TLC方法对通脉活血颗粒中的丹参、玄参、木香和甘草进行定性鉴别;采用HPLC法对通脉活血颗粒中的丹参酮ⅡA进行含量测定,使用Phenomenex Gemini C18色谱柱(250 mm×4.6 mm,5μm),以水-甲醇-乙腈(35∶35∶30)为流动相,流速1 mL.min-1,检测波长270 nm,柱温30℃。结果:丹参、玄参、木香和甘草的TLC鉴别斑点清晰,专属性强;HPLC法测定,丹参酮ⅡA进样量在16.3～652.8μg范围内与峰面积呈良好的线性关系(r=0.9999);平均回收率(n=6)为99.7%,RSD=2.0%。结论:所建立的方法可用于通脉活血颗粒的定性、定量检测。
Objective: To establish a qualitative and quantitative method for Tongmai Huoxue granule. Methods: The TLC method was used to qualitatively identify Radix Salviae Miltiorrhizae, Radix Scrophulariae, Magnolia officinalis and Glycyrrhiza uralensis Fisch. The content of tanshinone ⅡA in Tongmai Huoxue granule was determined by HPLC. The chromatographic separation was performed on a Phenomenex Gemini C18 column (250 mm × 4.6 mm, 5μm). The mobile phase was water-methanol-acetonitrile (35:35:30) with a flow rate of 1 mL.min-1. The detection wavelength was 270 nm and the column temperature was 30 ℃. Results: TLC identification spots of Radix Salviae Miltiorrhizae, Radix Scrophulariaceae, Radix Aconitum and Radix Glycyrrhizae were clear and highly specific. HPLC analysis showed that Tanshinone ⅡA injection had a good linear relationship with the peak area in the range of 16.3 ~ 652.8μg (r = 0.9999) ; Average recovery (n = 6) was 99.7%, RSD = 2.0%. Conclusion: The established method can be used for qualitative and quantitative detection of Tongmai Huoxue Granule.