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尖孢镰刀菌西瓜专化型〔FusariumoxysporumSchl.f.sp.niveum(E.F.Smith)SnyderetHansen〕菌株在4种培养基(理查德培养基,Armstrong镰刀菌培养基,MS无机盐+蔗糖培养基,PDB培养液)中均能产生镰刀菌酸(Fusaricacid),理查德培养基中镰刀菌酸最高时的浓度是其它3种培养基的6~22倍。理查德培养基以C/N为5∶1,葡萄糖为C源,NO-3为N源的营养条件较有利于镰刀菌酸产生。培养基初始pH值为4,通气和连续光照的培养条件最有利于菌株产生镰刀菌酸,烟酸的加入可终止镰刀菌酸产生。各培养基中孢子浓度与镰刀菌酸浓度之间呈负相关的变化趋势,培养基的pH值呈趋中(pH6~8)变化,与镰刀菌酸产生无相关性。在理查德培养基中,菌丝生长与镰刀菌酸产生之间存在平行关系
Fusarium oxysporum watermelon [Fusariumoxysporum Schl. f. sp. The strain niveum (E.F. Smith) Snyderet Hansen was able to produce Fusaricacid (Fusaric acid) in all four media (Richard medium, Armstrong medium, MS inorganic salt + sucrose medium, PDB medium) ), The highest concentration of Fusaric acid in Richard medium is 6 ~ 22 times of the other three kinds of medium. Richard medium with C / N ratio of 5: 1, glucose as C source and NO-3 as N source is more conducive to the production of Fusaric acid. The initial pH value of the medium was 4, and the culture conditions of aeration and continuous light were the best for the strain to produce Fusaric acid. The addition of niacin could stop the Fusaric acid production. There was a negative correlation between spore concentration and Fusaric acid concentration in each culture medium. The pH value of medium varied with pH6-8, and there was no correlation with Fusaric acid. In Richard medium, there is a parallel relationship between mycelial growth and Fusaric acid production