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目的:观察当归对单侧输尿管梗阻(UUO)大鼠肾小管间质纤维化过程中黏附分子P-选择素(P-selectin)和细胞间黏附分子-1(ICAM-1)、转化生长因子-β1(TGF-β1)等细胞因子的表达和CD68单核/巨噬细胞浸润的影响。方法:采用大鼠模型,52只SD大鼠分为:假手术组、UUO组、当归组。术后第7d和第14d留取标本处死大鼠,用免疫组化方法检测肾组织内P-selectin、ICAM-1、CD68、TGF-β1的表达。结果:与假手术组相比,UUO组大鼠第7d和第14d肾组织内ICAM-1、P-selectin、CD68、TGF-β1的表达均显著上调(P<0.05)。与UUO组相比,当归组两个时间段肾小管间质病变减轻,且肾组织内ICAM-1、CD68、TGF-β1的表达受到显著抑制(P<0.05)。结论:当归可能是通过抑制ICAM-1的表达和炎细胞的组织浸润,从而降低了肾小管间质TGF-β1的水平和纤维化的进展。
Objective: To observe the effects of Angelica on P-selectin and ICAM-1, TGF-β1 in tubulointerstitial fibrosis in rats with unilateral ureteral obstruction (UUO) β1 (TGF-β1) and other cytokines and CD68 monocyte / macrophage infiltration. Methods: Using rat model, 52 SD rats were divided into sham operation group, UUO group and Angelica group. The rats were sacrificed on the 7th day and the 14th day after operation, and the expression of P-selectin, ICAM-1, CD68 and TGF-β1 in renal tissues was detected by immunohistochemistry. Results: Compared with the sham operation group, the expression of ICAM-1, P-selectin, CD68 and TGF-β1 in the UUO group was significantly increased on the 7th and 14th day (P <0.05). Compared with UUO group, tubulointerstitial lesion was relieved in both groups and the expression of ICAM-1, CD68 and TGF-β1 in renal tissue was significantly inhibited (P <0.05). Conclusion: Angelica sinensis can decrease the level of TGF-β1 and the progress of fibrosis by inhibiting the expression of ICAM-1 and the infiltration of inflammatory cells.