论文部分内容阅读
目的观察三七总甙(PNS)对人增生性瘢痕成纤维细胞(HSF)向肌成纤维细胞转分化的作用,探讨PNS抗瘢痕纤维化的机制。方法体外培养HSF,采用不同浓度的PNS进行干预,根据细胞培养所加PNS浓度分为400μg/ml组、800μg/ml组及空白对照组(不加PNS)。采用细胞三维培养法检测HSF凝胶收缩情况,计算其收缩指数;免疫细胞化学染色法检测HSF中α平滑肌肌动蛋白(α-SMA)的表达;流式细胞仪检测HSF中α-SMA阳性细胞率。结果400μg/ml、800μg/ml组各时相点的胶原凝胶块收缩程度明显减轻,其收缩指数均小于空白对照组(P<0.05或P<0.01)。HSF中α-SMA阳性表达颗粒在细胞质内呈弥漫性分布;空白对照组HSF中α-SMA的阳性表达明显强于400μg/ml、800μg/ml组。400μg/ml、800μg/ml组α-SMA的阳性细胞率(31.52%、24.28%)均明显低于空白对照组(45.74%,P<0.05)。400μg/ml、800μg/ml组α-SMA的阳性细胞染色强度积分均明显低于空白对照组(P<0.05或P<0.01)。结论PNS能够抑制HSF向肌成纤维细胞的转分化,具有体外抗瘢痕纤维化的作用。
Objective To observe the effect of Panax notoginseng saponins (PNS) on the transdifferentiation of human hypertrophic scar fibroblasts (HSF) to myofibroblasts and explore the mechanism of PNS anti-scarring fibrosis. Methods HSF was cultured in vitro and PNS was used to intervene. According to the cell culture, PNS concentration was divided into 400 μg/ml group, 800 μg/ml group and blank control group (without PNS). The shrinkage index of HSF gel was measured by cell three-dimensional culture method, and the contraction index was calculated. The expression of α-SMA in HSF was detected by immunocytochemical staining; the α-SMA positive cells in HSF were detected by flow cytometry. rate. Results The degree of contraction of the collagen gel block at each time point of the 400 μg/ml and 800 μg/ml groups was significantly reduced, and the contraction index of the collagen gel blocks was smaller than that of the blank control group (P<0.05 or P<0.01). The positive expression of α-SMA in HSF was diffusely distributed in the cytoplasm; the positive expression of α-SMA in HSF was significantly stronger in the blank control group than in the 400 μg/ml and 800 μg/ml groups. The positive rates of α-SMA in the 400μg/ml and 800μg/ml groups were significantly lower than those in the blank control group (45.74%, P<0.05) (31.52% and 24.28%, respectively). The staining intensity of positive cells in 400μg/ml and 800μg/ml α-SMA groups was significantly lower than that in the blank control group (P<0.05 or P<0.01). Conclusion PNS can inhibit the transdifferentiation of HSF into myofibroblasts and has anti-scarring fibrosis in vitro.