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目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)联合低剂量顺铂(DDP)对食管癌EC9706细胞增殖的影响。方法单用DDP和单用TRAIL作用于EC9706细胞24、48、72 h后,利用MTT法检测各处理因素对EC9706细胞增殖的影响;TRAIL联合低剂量DDP作用48 h后,利用MTT法检测各处理因素对EC9706细胞增殖的影响。结果随着单用DDP和单用TRAIL浓度的提高,作用时间的延长,其对EC9706细胞的抑制作用增强。TRAIL与低剂量DDP联用可明显抑制EC9706细胞的增殖,与单用DDP或单用TRAIL相比,细胞存活率显著降低;联合DDP组和DDP+TRAIL组细胞增殖率均随着DDP浓度的增加而下降。结论TRAIL与低剂量DDP联合应用,可以抑制食管癌EC9706细胞的增殖,TRAIL能增强食管癌细胞对DDP的敏感性。
Objective To investigate the effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and low-dose cisplatin (DDP) on the proliferation of esophageal carcinoma EC9706 cells. Methods After treated with DDP and TRAIL alone for 24, 48 and 72 h, MTT assay was used to detect the effect of various treatment factors on the proliferation of EC9706 cells. After treated with TRAIL for 48 h, Effect of Factors on Proliferation of EC9706 Cells. Results With the increase of concentration of single DDP and single use of TRAIL, the inhibitory effect on the EC9706 cells was enhanced by prolonging the action time. In combination with low dose of DDP, TRAIL significantly inhibited the proliferation of EC9706 cells. Compared with DDP alone or TRAIL alone, the cell survival rate was significantly decreased. The proliferation rate of both DDP group and DDP + TRAIL group increased with the increase of DDP concentration And down. Conclusion TRAIL combined with low dose of DDP can inhibit the proliferation of EC9706 cells and TRAIL can enhance the sensitivity of esophageal carcinoma cells to DDP.