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以植物病原细菌黄单胞菌的avrXa7基因和ahpC基因的2个突变体及其野生型菌株为研究对象,探讨植物病原细菌内源过氧化氢水平与菌株毒性的联系。AR/HRP法分析病原菌的过氧化氢清除力,组织化学法对细胞内过氧化氢进行定量和定位分析。结果表明,avrXa7基因的突变诱导病原菌毒性的显著降低,并引起病原菌内源过氧化氢积累水平显著下降;ahpC基因的突变诱导病原菌内源过氧化氢水平显著降低,但未能引起病原菌毒性产生显著的变化。研究结果说明,在植物病原细菌黄单胞病菌的致病机制中,病原菌内源过氧化氢的积累水平并不能直接决定菌株的毒性,而是处在avrXa7基因的下游,受到avrXa7或者更多毒性相关基因的调控,参与病原菌的致病过程。
Two strains of avrXa7 and ahpC gene of Xanthomonas oryzae and their wild type strains were used to investigate the relationship between the level of endogenous hydrogen peroxide in plant pathogenic bacteria and the strain toxicity. AR / HRP analysis of pathogenic bacteria hydrogen peroxide scavenging capacity, histochemistry of intracellular hydrogen peroxide quantitative and location analysis. The results showed that the avrXa7 gene mutation induced a significant reduction in pathogenic bacteria toxicity and caused a significant decrease in the accumulation of endogenous hydrogen peroxide in the pathogenic bacteria. The mutation of the ahpC gene induced a significant decrease in the level of endogenous hydrogen peroxide in the pathogenic bacteria but failed to cause significant virulence in the pathogenic bacteria The change. The results show that in the pathogenic mechanism of Xanthomonas oryzae, the level of endogenous hydrogen peroxide accumulation in pathogenic bacteria does not directly determine the virulence of the strain, but is downstream of the avrXa7 gene and is affected by avrXa7 or more Related gene regulation, involved in pathogenesis of pathogenic bacteria.