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目的:从大头金蝇幼虫中分离纯化天然抗菌肽,并检测抗菌肽的抑菌活性。方法:提取大头金蝇幼虫抗菌肽,粗提物经凝胶过滤色谱、阳离子交换树脂、反相色谱纯化,纯化产物检测其抑菌活性并利用GFC-HPLC测定其分子量。结果:最终纯化出一个活性组分,该组分对铜绿假单胞菌的最小抑菌浓度为20.40μg/m L,对金黄色葡萄球菌的最小抑菌浓度为2.04 mg/m L,对大肠杆菌的最小抑菌浓度为0.17 mg/m L;该活性组分分子量大小为2570 u。结论:该抗菌肽组分对铜绿假单胞菌有较强抑菌活性,有较好的开发利用价值。
OBJECTIVE: To isolate and purify natural antibacterial peptides from the golden flies larvae, and to examine the antibacterial activity of the antibacterial peptides. Methods: Antimicrobial peptides were extracted from the larvae of S.japonicus. The crude extracts were purified by gel filtration chromatography, cation exchange resin and reverse phase chromatography. The antibacterial activity of the purified products was tested by GFC-HPLC. Results: The final purification of an active ingredient, the components of Pseudomonas aeruginosa minimum inhibitory concentration of 20.40μg / m L, the minimum inhibitory concentration against Staphylococcus aureus was 2.04 mg / m L, the large intestine The minimum inhibitory concentration of bacilli was 0.17 mg / m L; the molecular weight of the active ingredient was 2570 u. Conclusion: The antibacterial peptide has strong antibacterial activity against Pseudomonas aeruginosa, and has good development and utilization value.