用植物组织培养物进行生物转化

• 作 者: 谢启昆
• 出 处: 国外医学参考资料.药学分册
• 发表日期: 1977年01期
• 关键词: 毒芹 培养物 长春花 roseus 植物组织 代谢产物 浓氨水 乙酚 乙酸乙醋 高压蒸汽灭菌

作者研究了三种植物悬浮培养物转化几种芳香族化合物的能力,并鉴定了转化产物。悬浮培养物组织取自下列三种植物:长春花(Catharanthus roseus);磁麻(Apocy-num cannabinum)和毒芹(Conium macu-latum)。培养基:每一种组织选用其最适宜的培养基。长春花用PRL配方,加1毫克/升2,4-D;磁麻用改进的Murashige培养基,其中含10％椰子汁和1毫克/升2,4-D;毒芹用

The authors studied the ability of three plant suspension cultures to convert several aromatic compounds and identified conversion products. Suspension culture tissues were taken from the following three plants: Catharanthus roseus; Apocy-num cannabinum and Conium macu-latum. Media: Choose the most appropriate medium for each tissue. Catharanthus roseus with PRL formula, plus 1 mg / l 2,4-D; Magna; with improved Murashige medium containing 10% coconut milk and 1 mg / l 2,4-D;