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目的探讨唐草片对化疗药物引起的小鼠白细胞减少症的防治作用。方法成年健康小鼠60只,随机分为正常对照组、模型组、低剂量组、中剂量组、高剂量组和阳性药物对照组,每组10只。唐草片低、中、高剂量组分别灌服0.5、1.0、2.0g/kg唐草片,模型组、正常对照组灌服0.1mL/10g水。5d后除正常对照组外,每只动物腹腔注射3.0g/kg丝裂霉素C,第10天,再次腹腔注射2.0g/kg丝裂霉素C,阳性药物对照组在造模成功后皮下注射重组人粒细胞集落刺激因子50μg/kg,连续注射4d。各组在完成2次丝裂霉素C注射后,每周测定1次外周血细胞。实验结束时取各组小鼠一侧股骨骨髓细胞,培养14d后计数红细胞集落生成单位、粒细胞-巨噬细胞集落生成单位、粒细胞-红细胞-巨噬细胞-巨核细胞集落生成单位数量,取另一侧股骨骨髓细胞,计数有核细胞数。结果注射丝裂霉素C后24d时,中、低剂量组和阳性药物对照组白细胞计数[(9.91±2.73)×109/L、(10.00±3.10)×109/L、(9.68±3.19)×109/L]明显高于模型组[(6.92±1.61)×109/L](P<0.05),与正常对照组[(9.60±2.07)×109/L]比较差异无统计学意义(P>0.05);40d时,低剂量组白细胞计数[(15.13±2.41)×109/L]高于模型组[(10.06±2.17)×109/L](P<0.05);33d时,低剂量组红细胞数[(10.47±0.57)×1012/L]高于模型组[(9.10±1.15)×1012/L](P<0.05);低剂量组红细胞集落生成单位[(29.0±9.9)个/105]、粒细胞-巨噬细胞集落生成单位[(31.0±9.9)个/105]克隆数明显高于模型组[(6.5±0.7)、(17.5±2.1)个/105](P<0.05),中剂量组粒细胞-红细胞-巨噬细胞-巨核细胞集落生成单位克隆数[(16.0±2.8)个/105]明显高于模型组[(8.0±1.4)个/105](P<0.05)。结论唐草片对丝裂霉素C造成的白细胞降低模型小鼠有升高白细胞计数、保护造血功能的作用。
Objective To investigate the prevention and treatment of Tangcao Tablet on leukemia mice caused by chemotherapy drugs. Methods Sixty adult healthy mice were randomly divided into normal control group, model group, low dose group, middle dose group, high dose group and positive drug control group, with 10 rats in each group. Tang, low, medium and high dose groups were fed with 0.5,1.0,2.0 g / kg Tangcao tablets, the model group, normal control group fed with 0.1mL / 10g water. After 5 days, except for the normal control group, each animal was intraperitoneally injected with 3.0 g / kg mitomycin C, and on the 10th day, 2.0 g / kg mitomycin C was injected intraperitoneally again. The positive drug control group was injected subcutaneously Injection of recombinant human granulocyte colony-stimulating factor 50μg / kg, continuous injection 4d. Peripheral blood cells were measured once a week after the completion of two injections of mitomycin C in each group. At the end of the experiment, one side of the femur bone marrow cells of each group was taken and counted the numbers of erythrocyte colony forming units, granulocyte-macrophage colony forming units and granulocyte-erythrocyte-macrophage-megakaryocyte colony forming units after 14 days of culture Femoral bone marrow cells on the other side, counting the number of nucleated cells. Results After 24 hours of MMC injection, the white blood cell counts (9.91 ± 2.73) × 109 / L, (10.00 ± 3.10) × 109 / L and (9.68 ± 3.19) × 109 / L] was significantly higher than that of the model group [(6.92 ± 1.61) × 109 / L] (P <0.05), but no significant difference compared with the normal control group [(9.60 ± 2.07) × 109 / L] 0.05). At 40 days, the white blood cell count of low dose group (15.13 ± 2.41) × 109 / L was higher than that of model group [(10.06 ± 2.17) × 109 / L, P < (10.47 ± 0.57) × 1012 / L were higher than those in model group [(9.10 ± 1.15) × 1012 / L] (P <0.05). The number of erythrocyte colony forming units in low dose group was (29.0 ± 9.9) / 105 (31.0 ± 9.9) / 105], the number of colony-forming units of granulocyte-macrophage was significantly higher than that of model group [(6.5 ± 0.7), (17.5 ± 2.1) /105)(P<0.05) The number of cloned granulocyte-erythrocyte-macrophage-megakaryocyte colony forming units in the dose group [(16.0 ± 2.8) / 105] was significantly higher than that in the model group [(8.0 ± 1.4) /105)(P<0.05). Conclusion Tangcao tablet has the effect of increasing leukocyte count and protecting hematopoietic function in leukopenia mice induced by mitomycin C.