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目的:观察活性水对模拟失重大鼠内毒素引起微循环障碍模型血液流变学与微循环的影响。方法:取健康雄性SPF级Wistar大鼠50只,随机分为地面对照组、地面模型组、尾吊模型组、尾吊模型+市售某活性水组、尾吊模型+自制活性水组5组,前3组分别自由饮用纯净水,后2组分别自由饮用市售某活性水、自制活性水30d后,地面对照组腹腔注射注射用水,其余各组均由腹腔注入内毒素5mg/kg体重造成炎症刺激引起微循环障碍模型后进行实验。造模完成60min后由心脏取血,采用赛科希德SA-6000全自动血流变仪检测血液流变学指标。结果:与地面模型组相比,尾吊模型组的红细胞变形指数降低,血液黏度、红细胞聚集指数等指标均有升高的趋势,说明模拟失重状态下可出现一定程度的血液流变学障碍。与尾吊模型组相比,尾吊大鼠饮用自制活性水30d后能明显降低模拟失重微循环障碍大鼠低切全血黏度、降低红细胞聚集指数(P<0.05),并且使其达到地面正常水平(P>0.05);对红细胞变形性无明显影响,这与血液黏度的变化是一致的。此外,市售某活性水对模拟失重大鼠微循环障碍模型血液流变学与微循环的影响效果不明显。结论:自制活性水能较好地改善由内毒素引起机体血液黏度、流动性和凝滞性的变化,自制活性水具有一定的改善模拟失重大鼠微循环障碍的作用。
Objective: To observe the effect of active water on the hemorrheology and microcirculation of endotoxin-induced microcirculation disturbance in simulated weightless rats. Methods: Fifty healthy Wistar male SPF rats were randomly divided into five groups: ground control group, ground model group, tail-suspended model group, tail-hanging model + commercial active water group, tail-hanging model + homemade active water group , The first three groups were free to drink purified water, the latter two groups were free to drink a commercially available water, homemade active water 30d after the ground control group intraperitoneal injection of water for injection, and the remaining groups by the peritoneal injection of endotoxin 5mg / kg weight Inflammation stimulates the model of microcirculation after the experiment. Blood was collected from the heart after 60 minutes of modeling, and hemorheological indexes were measured by Saikohide SA-6000 automatic blood rheometer. Results: Compared with the ground model group, the index of erythrocyte deformability decreased and the indexes of blood viscosity and erythrocyte aggregation index tended to increase, which indicated that there was a certain degree of hemorrheological disorder in simulated weightlessness. Compared with the tail-hang model group, tail-dwelling rats drinking low-cut whole blood viscosity and reducing the erythrocyte aggregation index (P <0.05) after drinking 30d of active water for 30 days in simulated weightlessness and microcirculation rats, Level (P> 0.05); no significant effect on erythrocyte deformability, which is consistent with changes in blood viscosity. In addition, the effect of commercially available water on the hemorheology and microcirculation of microcirculation model in simulated weightless rats was not obvious. CONCLUSION: Self-made active water can improve the changes of blood viscosity, fluidity and blood stagnation caused by endotoxin. Self-made active water can improve the microcirculation disturbance of simulated weightless rats.