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目的:探讨地塞米松对顺铂诱导HepG2细胞凋亡的影响,并观察Bcl-2和Caspase-3蛋白的表达。方法:HepG2细胞与顺铂及不同浓度的地塞米松共培养,RT-PCR检测Bcl-2、Caspase-3的mRNA表达,Western blot检测Bcl-2、Caspase-3蛋白表达,流式细胞术(FACS)检测各组HepG2细胞的凋亡情况。结果:随着地塞米松浓度的增加,HepG2细胞中Bcl-2蛋白表达量升高,Caspase-3蛋白表达量下降。同时细胞凋亡率降低。结论:地塞米松部分通过Bcl-2途径抑制肝癌细胞的凋亡,而Caspase-3又在调控细胞凋亡中起重要作用。
Objective: To investigate the effect of dexamethasone on the apoptosis of HepG2 cells induced by cisplatin, and to observe the expression of Bcl-2 and Caspase-3 protein. Methods: HepG2 cells were co-cultured with cisplatin and dexamethasone at different concentrations. The mRNA expressions of Bcl-2 and Caspase-3 were detected by RT-PCR. The expressions of Bcl-2 and Caspase-3 were detected by Western blot. FACS) to detect the apoptosis of HepG2 cells in each group. Results: With the increase of dexamethasone concentration, the expression of Bcl-2 protein and the expression of Caspase-3 protein decreased in HepG2 cells. While the rate of apoptosis decreased. Conclusion: Dexamethasone partially inhibits hepatocellular carcinoma cell apoptosis through the Bcl-2 pathway, and Caspase-3 plays an important role in the regulation of apoptosis.