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目的运用生物信息学方法分析微小巴贝斯虫烯醇化酶(BmEno)基因编码蛋白的主要特征和抗原表位。方法运用ORF Finder、PROSITE、ProtPar am、SignalP、TMpred、SOSUI、MotifScan、Bcepred、HNN等生物信息学在线分析软件,结合DNASTAR生物信息学分析软件,预测BmEno蛋白的开放阅读框、特征结构域、理化性质、信号肽、跨膜区、翻译后修饰位点、可溶性、亲水性、表面可及性、二级结构和抗原表位。结果该蛋白由438个氨基酸组成,在349-362位存在烯醇化酶的特征结构域,分子式为C_(2098)H_(3366)N_(570)O_(648)S_(18),分子质量单位为47.5203ku,等电点理论值为5.98,有25个翻译后修饰位点,10个亲水性参数得分≥1.9的区域,6个柔韧性参数得分≥2.0的区域,18个表面可及性参数得分≥1.9的区域,7个潜在的B细胞抗原表位,15个潜在的T细胞抗原表位,为可溶性蛋白。结论微小巴贝斯虫烯醇化酶蛋白为可溶性蛋白,有多个抗原表位,具有潜在的免疫原性,可以作为微小巴贝斯虫的疫苗候选抗原。
Objective To analyze the main features and antigenic epitopes of BmEno gene coded by bioinformatics method. Methods Bioinformatics software such as ORF Finder, PROSITE, ProtPar am, SignalP, TMpred, SOSUI, MotifScan, Bcepred and HNN was used to analyze the open reading frame of BmEno protein, combined with DNASTAR bioinformatics analysis software. Nature, signal peptide, transmembrane region, posttranslational modification site, solubility, hydrophilicity, surface accessibility, secondary structure and antigenic epitope. Results The protein consisted of 438 amino acids with a characteristic enolase at 349-362. The molecular formula was C 2098 H 3366 N 570 570 64. The molecular mass units were 47.5203 ku, the theoretical isoelectric point was 5.98, there were 25 posttranslational modification sites, 10 regions with hydrophilic parameter score≥1.9, 6 regions with score ≥2.0, 18 surface accessibility parameters Scores ≥1.9, seven potential B cell epitopes and fifteen potential T cell epitopes were soluble proteins. Conclusion The micro-Babesia enolase protein is a soluble protein with multiple epitopes and potential immunogenicity, which can be used as a vaccine candidate antigen for Babesia minibi.