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采用沙培的方法,以最适锰浓度为对照,设置重度锰缺乏(对照浓度的1/25,S1表示)、中度锰缺乏(对照浓度的1/5,S2表示)、中度锰过量(对照浓度的5倍,S3表示)、重度锰过量(对照浓度的25倍,S4表示)不同程度锰缺乏和锰过量处理,研究了锰胁迫对大豆氮代谢关键酶活性的影响。结果表明:不同程度锰缺乏胁迫抑制了叶片GS和GOGAT活性,而锰过量胁迫则起促进作用;锰缺乏胁迫对根GS无显著影响,锰过量胁迫则起抑制作用;不同程度锰胁迫对根GOGAT活性均起抑制作用,而对茎GS和GOGAT活性则无显著影响;重度锰胁迫(S1和S4)对叶片GDH起促进作用,轻度锰胁迫(S2和S3)起抑制作用;不同程度锰胁迫对茎和根GDH活性均起抑制作用。综合分析表明,大豆叶、茎、根GS、GOGAT、GDH活性对锰胁迫的响应不同,这可能与上述氮代谢相关酶在不同器官中的氮代谢功能、同工酶组成不同有关,并最终导致大豆氮代谢状况发生改变。
In the method of Sapiens, the optimal manganese concentration was used as the control, and the heavy manganese deficiency (1/25 of the control concentration, S1), moderate manganese deficiency (1/5 of the control concentration, S2), moderate manganese excess (5 times of the control concentration, S3), heavy manganese excess (25 times of the control concentration, S4) with different degrees of manganese deficiency and manganese overdose, the effects of Mn stress on the key enzyme activities of nitrogen metabolism in soybean were studied. The results showed that manganese deficiency inhibited the activities of GS and GOGAT, while manganese overdose could promote the growth of seedlings. Manganese deficiency had no significant effect on root GS and excessive Mn stress, however, While the activity of GS and GOGAT had no significant effect. Heavy manganese stress (S1 and S4) promoted the GDH activity of leaves and mild manganese stress (S2 and S3). Manganese with different degrees of Mn stress Both stem and root GDH activity were inhibited. Comprehensive analysis showed that the response of GS, GOGAT and GDH activities of soybean leaves to different Mn stress was different, which may be related to the nitrogen metabolism function and isoenzyme composition of different nitrogen metabolism related enzymes in different organs, and finally resulted in Soybean nitrogen metabolism changes.