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目的研究乙型肝炎肝硬化病人左右半肝形态及肝硬化病人肝组织标本的HE染色和肝硬化相关因子基质金属蛋白酶-2和碱性成纤维细胞生长因子-2的表达。方法对30例乙肝肝硬化致门脉高压征行脾切除术和乙肝肝硬化失代偿行肝移植手术病人行CT检查,手术中标本摄片,留取形态学资料。同时留取此30例病人肝组织标本,进行HE染色,根据肝硬化标准判断肝纤维化程度并行免疫组化染色检测MMP2和bFGF2的表达。结果1)发现右半肝萎缩较左半肝萎缩明显增多;2)HE染色亦显示右半肝肝硬化较左半肝肝硬化明显增多;3)MMP2在病例组左半肝的表达明显高于对照组左半肝表达,有统计学差异;4)bFGF2在病例组左半肝表达与对照组左半肝表达经检验无统计学差异。在病例组右半肝表达明显高于对照组右半肝表达,有统计学差异。结论肝硬化时左右半肝纤维化程度存在明显地差异性,为我们今后治疗肝硬化合并肝癌,手术切除肝组织大小及部位提供了新的理论依据。
Objective To study the hematoxylin and eosin (HE) staining and the expression of matrix metalloproteinase-2 and basic fibroblast growth factor-2 in cirrhotic patients with cirrhosis and cirrhosis. Methods CT scan of 30 patients undergoing hepatic cirrhosis with splenectomy due to portal hypertension and liver transplantation undergoing decompensation of hepatitis B cirrhosis were performed. Specimens were collected during operation and morphological data were collected. At the same time, 30 cases of liver tissue samples were collected for HE staining. The degree of hepatic fibrosis was determined according to the criteria of cirrhosis and the expressions of MMP2 and bFGF2 were detected by immunohistochemistry. Results 1) Found that the right hepatic atrophy significantly increased compared with the left hepatic atrophy; 2) HE staining also showed that the right hepatic cirrhosis than the left hepatic cirrhosis was significantly increased; 3) The expression of MMP2 in the left hemiliver in the case group was significantly higher than The expression of bFGF2 in the left half of the liver and the left half of the liver in the control group showed no statistical difference. The expression of right hemisphere in the case group was significantly higher than that in the control group, with statistical difference. Conclusion There is a significant difference in the degree of hepatic fibrosis between cirrhosis and cirrhosis, which provides a new theoretical basis for the future treatment of liver cirrhosis complicated with liver cancer and surgical resection of liver tissue size and location.