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目的 观察电应激对十二指肠组织形态及功能代谢的影响,旨在探讨多种疾病过程中肠病理学及病理生理学改变的一般机制。 方法 用电刺激复制应激模型,图象计量分析的方法进行组织学观察,化学方法检测组织内SOD,MDA和NO含量。 结果 应激组平滑肌及肠腺细胞核的面积、周长、最大直径、最小直径分别是(56.1±98.4)μm~2,(115.4±69.6)μm~2;(25.1±50.4)μm,(47.1±60.8)μm;(6.0±7.50)μm,(5.5±7.0)μm;(1.3±2.5)μm,(1.0±1.8)μm,与对照组相比均有显著性改变;组织内SOD,MDA和NO含量分别是(113.5±3.6)NU/mg蛋白,(1.93±0.15)mol/g蛋白和(0.313±0.086)μmol/g蛋白,较对照组明显增高(P<0.01或P<0.05)。 结论 电应激可明显改变十二指肠组织结构,其机制与组织内氧化机制的激活有关。
Objective To observe the effect of electrical stress on the morphology and functional metabolism of duodenum and to explore the general mechanism of intestinal pathology and pathophysiology in a variety of diseases. Methods The model of stress stimulation was established by electrical stimulation, the histological observation was performed by means of image quantitative analysis, and the contents of SOD, MDA and NO were detected by chemical methods. Results The area, perimeter, maximum diameter and minimum diameter of smooth muscle and glandular cell nucleus of stress group were (56.1 ± 98.4) μm ~ 2, (115.4 ± 69.6) μm ~ 2, (25.1 ± 50.4) μm, (6.0 ± 7.50) μm, (5.5 ± 7.0) μm, (1.3 ± 2.5) μm and (1.0 ± 1.8) μm, respectively. Compared with the control group, the changes of SOD, MDA and NO (113.5 ± 3.6) NU / mg protein, (1.93 ± 0.15) mol / g protein and (0.313 ± 0.086) μmol / g protein were significantly increased compared with the control group (P <0.01 or P <0.05). Conclusion Electrical stress can significantly change the structure of duodenum, and its mechanism is related to the activation of the oxidation mechanism in the tissue.