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目的观察日本血吸虫紫外线致弱尾蚴疫苗(UVC)免疫动物攻击感染后的局部组织免疫病理变化。方法将70只C57BL/6小鼠随机分为疫苗免疫组和感染对照组。疫苗组小鼠接种紫外线致弱日本血吸虫尾蚴后5周,再经腹部皮肤攻击感染正常日本血吸虫尾蚴(800±50)条;感染对照组经皮肤感染同量尾蚴。于攻击感染后6～120h的不同时间点各剖杀小鼠5只,取攻击部位皮肤及/或肺组织,进行病理学观察。结果UVC疫苗免疫小鼠攻击感染日本血吸虫尾蚴后,皮肤炎症反应较感染对照出现早、反应强烈、持续时间长,EOS百分比高,但肺部出血斑点出现时间(72h)迟于感染对照组(48h)。72～120h,疫苗组小鼠肺部局灶性炎症明显,肉芽肿样结节形成,肺泡壁多正常,而感染对照组小鼠肺组织炎症轻,但肺泡壁水肿明显,且有较多红细胞渗出。结论紫外线致弱尾蚴疫苗免疫增强了小鼠皮肤及肺组织的细胞反应及其杀虫作用。
Objective To observe the immunopathological changes of local tissue after challenge with UVC immunized animal against Schistosoma japonicum. Methods Seventy C57BL / 6 mice were randomly divided into the vaccine-immunized group and the infected control group. The mice in the vaccine group were infected with cercariae of Schistosoma japonicum (800 ± 50) 5 weeks after inoculation of UV-attenuated Schistosoma japonicum cercariae, and the control group was infected with the same amount of cercariae through the skin. Five mice were sacrificed at different time points from 6 to 120 hours after challenge and the pathological examination was performed on the skin and / or lung tissue of the attack site. Results The UVC vaccine immunized mice infected with Schistosoma japonicum cercariae earlier than the infected controls. The response to the UVC vaccine was strong and persistent for a long time and the percentage of EOS was high. However, the onset of pulmonary hemorrhage was delayed (72h) ). 72 ~ 120h, the mice in the vaccine group had obvious focal lung inflammation, granulomatous nodules formed, and the alveolar wall was more normal. However, the mice in the infected control group had mild inflammation in the lung tissue but obvious edema in the alveolar wall with more erythrocytes Exudation. Conclusion Immunization with UV-induced weak cercariae vaccine enhanced cellular responses and insecticidal effects on mouse skin and lung tissues.