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[目的]探讨人参皂甙Rb1(GRb1)对香烟烟雾诱导大鼠神经细胞损伤的保护作用和机制。[方法]制备烟灌注大鼠模型,吸烟量分别为8、20、40支/d,12周后腹腔注射不同剂量的GRb1(10、20、40 mg/kg)。各组大鼠同时制备皮层神经细胞培养液,24 h后应用Annexin V联合流式细胞仪检测细胞凋亡率和坏死率,Western-Blot检测存活素(Survivin)的表达水平,Fura-2/AM负载及荧光标记检测神经细胞内游离Ca2+浓度。[结果]香烟烟雾刺激诱导神经细胞发生凋亡和坏死,与对照组相比凋亡率和坏死率显著增高。与对照组相比,8支/d香烟烟雾刺激使Survivin表达增强34%,20、40支/d香烟烟雾刺激使Survivin表达分别下降48%和67%;20、40 mg/kg的GRb1使细胞凋亡率分别下降30.6%和40.3%,细胞坏死率分别下降32.5%和39.4%,Survivin表达水平上调45.1%和54.8%。香烟烟雾可使神经细胞内游离Ca2+浓度显著升高,GRb1使神经细胞内Ca2+浓度显著下降(P﹤0.01)。[结论]GRb1通过抑制细胞内Ca2+超载,调节Survivin表达,减轻香烟烟雾对神经细胞的损伤。
[Objective] To investigate the protective effect and mechanism of ginsenoside Rb1 (GRb1) on cigarette smoke-induced neuronal injury in rats. [Method] The rat model of perfused rat was prepared. The smoking amount was 8, 20 and 40 per day respectively. Different doses of GRb1 (10, 20 and 40 mg / kg) were injected intraperitoneally 12 weeks later. After 24 h, the apoptotic rate and necrosis rate were detected by Annexin V and flow cytometry. Survivin expression was detected by Western-Blot. Fura-2 / AM Load and fluorescent labeling detection of intracellular free Ca2 + concentration. [Result] Cigarette smoke stimulation induced the apoptosis and necrosis of nerve cells. Compared with the control group, the apoptosis rate and necrosis rate were significantly increased. Compared with the control group, the expression of Survivin increased by 34% after 8 cigarettes / d cigarette smoke stimulation, the expression of Survivin decreased by 48% and 67% respectively with 20,40 cigarettes / d cigarette smoke stimulation; The apoptotic rate decreased by 30.6% and 40.3% respectively. The cell necrosis rate decreased by 32.5% and 39.4% respectively, and the Survivin expression level increased by 45.1% and 54.8% respectively. Cigarette smoke increased the concentration of free Ca2 + in nerve cells significantly, while GRb1 decreased the concentration of Ca2 + in nerve cells significantly (P <0.01). [Conclusion] GRb1 can reduce the damage of nerve cells by cigarette smoke by inhibiting the overload of intracellular Ca2 + and regulating the expression of Survivin.