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目的:研究清脑宣窍滴丸对急性脑缺血损伤的保护作用及机理。方法:采用线栓法所致大鼠大脑中动脉缺血再灌注(MCAO)模型,测定大鼠脑梗塞范围;测定脑匀浆中乳酸(LD)、丙二醛(MDA)、谷胱甘肽(GSH)含量及乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活力;应用免疫组化染色的方法,观察清脑宣窍滴丸对黏附分子-1(ICAM-1)表达的影响。结果:清脑宣窍滴丸180 mg.kg-1、90 mg.kg-1可明显减小MCAO大鼠脑梗塞范围;180 mg.kg-1可明显降低MCAO大鼠患侧脑匀浆中LD含量,180 mg.kg-19、0 mg.kg-1、45 mg.kg-1可明显降低手术侧大脑组织中升高的LDH活力、提高SOD活力、降低MDA含量,180 mg.kg-1、90 mg.kg-1可明显提高手术侧大脑组织中GSH含量;180 mg.kg-1可显著降低ICAM-1阳性表达的积分光密度(IOD)。结论:清脑宣窍滴丸对脑缺血有保护作用,可能机理是通过减轻酸中毒、抑制自由基损伤、恢复自由基代谢平衡,抑制黏附分子表达来减轻缺血再灌注后炎症反应。
Objective: To study the protective effects and mechanisms of Qingnao Xuanqiao Drop Pills on acute cerebral ischemia injury. Methods: The middle cerebral artery occlusion (MCAO) model induced by thread occlusion was used to determine the extent of cerebral infarction in rats. The content of lactate (LD), malondialdehyde (MDA), glutathione (GSH), lactate dehydrogenase (LDH) and superoxide dismutase (SOD) were measured by immunohistochemical method. The expression of ICAM-1 influences. Results: Qingnaoganjiao drop pills 180 mg.kg-1, 90 mg.kg-1 can significantly reduce the range of cerebral infarction in MCAO rats; 180 mg.kg-1 can significantly reduce MCAO rat ipsilateral brain homogenate LD, 180 mg.kg-19,0 mg.kg-1, 45 mg.kg-1 can significantly reduce the activity of LDH in the operation side of the brain tissue, increase the activity of SOD, reduce the content of MDA, 180 mg.kg- 1,90 mg.kg-1 could significantly increase GSH content in the operation-side brain tissue; 180 mg.kg-1 could significantly reduce the integrated optical density (IOD) of ICAM-1 positive expression. Conclusions: Qingnao Xuanqiao dripping pill has a protective effect on cerebral ischemia. The possible mechanism is to alleviate the inflammatory reaction after ischemia-reperfusion by reducing acidosis, inhibiting free radical damage, restoring the balance of free radical metabolism and inhibiting the expression of adhesion molecules.