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目的：探讨中性粒细胞（ＰＭＮ）在急性肺损伤（ＡＬＩ）发生中的作用及ＩＬ－１０对ＡＬＩ的拮抗作用。方法：用 ＬＰＳ（１００ μｇ／只）或 ＬＰＳ＋ ＩＬ－１０（１μｇ／只）向ＳＤ大鼠气管内滴注复制ＡＬＩ模型，检测支气管肺泡灌洗液（ＢＡＬＦ）中ＰＭＮ数目、蛋白质及丙二醛（ＭＤＡ）含量，并进行组织学观察。结果：ＬＰＳ气管内滴注可引起ＢＡＬＦ中ＰＭＮ数目明显增加，伴有蛋白质及 ＭＤＡ含量的增高，光镜观察显示肺组织间隙弥漫性炎细胞浸润。 ＬＰＳ＋ＩＬ－１０组则ＢＡＬＦ中ＰＭＮ数目、蛋白质及ＭＤＡ含量显著低于ＬＰＳ组，肺组织中ＰＭＮ浸润程度也明显轻。结论：ＰＭＮ在ＡＬＩ发病中具有重要作用。ＩＬ－１０能够拮抗ＬＰＳ所致ＡＬＩ的发生。
Objective: To investigate the role of neutrophils (PMN) in the pathogenesis of acute lung injury (ALI) and the antagonism of IL-10 on ALI. METHODS: Duplicate ALI model was induced by intratracheal instillation of LPS (100 μg / L) or LPS + IL-10 (1 μg / L) to detect the number of PMN, protein and malondialdehyde in bronchoalveolar lavage fluid (MDA) content, and histological observation. Results: Intratracheal instillation of LPS caused a significant increase in the number of PMN in BALF, accompanied by an increase in the content of protein and MDA, and diffuse inflammatory cell infiltration in the interstitial lung space was observed by light microscopy. The number of PMN, protein and MDA in BALF in LPS + IL-10 group were significantly lower than that in LPS group, and the PMN infiltration in lung tissue was also significantly lower. Conclusion: PMN plays an important role in the pathogenesis of ALI. IL-10 can antagonize the LPS-induced ALI.