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利用8对多态性较好的SRAP引物对海南120份普通野生稻、55份疣粒野生稻和26份药用野生稻进行扩增,在检测到的219个位点中,普通野生稻的多态性位点率为74.89%,疣粒野生稻为42.47%,药用野生稻25.11%。Shannon多样性指数以普通野生稻最高为0.3277,疣粒野生稻为0.2044,药用野生稻最低为0.1113。UPGMA聚类分析结果显示供试材料与地理来源相一致,相关性强,各居群个体间没有出现任何交叉。普通野生稻总群体基因多样性为0.2135,群体内基因多样性显著高于居群间基因多样性,说明普通野生稻居群遗传分化不显著,遗传多样性主要来自于居群内。基于Nei’s基因多样度和Shannon多样性指数特点,认为实施保护策略时,优先保护遗传多样性最丰富的WDL和WDA居群。疣粒野生稻居群存在中等程度的遗传分化,建议原生境保护;药用野生稻居群数量较少,建议原生境保护。
Eight pairs of SRAP primers with good polymorphism were used to amplify 120 wild O. sativa, 55. wart wild and 26 wild O. officinalis. Among the 219 loci detected, Polymorphic site rate was 74.89%, warty wild rice was 42.47% and medicinal wild rice was 25.11%. Shannon’s diversity index was 0.3277 for common wild rice, 0.2044 for wild wart and 0.1113 for medicinal wild rice. UPGMA cluster analysis showed that the tested materials were consistent with the geographical origin, strong correlation and no crossover between populations. The total gene diversity of Oryza rufipogon (Oryza sativa) was 0.2135, and the genetic diversity of Oryza rufipogon populations was significantly higher than that of Oryza rufipogon (Oryza sativa L.), which indicated that the genetic differentiation of Oryza rufipogon (Oryza rufipogon) was not significant and the genetic diversity mainly came from within populations. Based on the features of Nei’s gene diversity and Shannon’s diversity index, it is considered that WLS and WDA populations with the most abundant genetic diversity should be given priority when implementing conservation strategies. Warty wild rice populations there is a moderate degree of genetic differentiation, the proposed in situ conservation; medicinal wild rice population less recommended in situ conservation.