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应用免疫组织化学方法对河南省食管癌高发区林州市居民贲门癌原发病灶和淋巴结转移病灶肿瘤抑制基因Ｐ５３和肿瘤转移抑制基因ｎｍ２３的变化进行研究。结果：在３７例贲门腺癌中，１８例原发病灶和转移病灶配对组织中一致性Ｐ５３免疫阳性反应的发生率为６７％（１２／１８）；定量分析表明，原发病灶中Ｐ５３蛋白聚集阳性细胞明显高于淋巴结转移病灶中Ｐ５３的表达；Ｐ５３免疫阳性的贲门腺癌淋巴结转移率（７８％）明显高于Ｐ５３免疫阴性的贲门腺癌淋巴结转移率（２９％，Ｐ＜０．０５）。３７例贲门癌组织中，ｎｍ２３免疫阴性者为２４例，占６５％，ｎｍ２３阴性者淋巴结转移率（７５％）明显高于ｎｍ２３表达阳性者淋巴结转移率（３１％，Ｐ＜０．０５）。在１０例贲门癌淋巴结转移组织中，淋巴结周边部转移病灶Ｐ５３阳性细胞数明显高于中心部。提示：Ｐ５３和ｎｍ２３在贲门癌转移过程中起一定作用。
The immunohistochemical method was used to study the changes of tumor suppressor gene P53 and metastasis suppressor gene nm23 in primary cancer and lymph node metastases in residents of Linzhou City, a high incidence area for esophageal cancer in Henan Province. RESULTS: In 37 cases of cardiac adenocarcinoma, the coincident rate of P53 immunopositive reaction was 18% (12/18) in matched tissues of 18 primary lesions and metastatic lesions; quantitative analysis showed that P53 protein aggregated in primary lesions. The number of positive cells was significantly higher than that of lymph node metastatic lesions; the rate of lymph node metastasis of gastric cardia adenocarcinoma positive for P53 (78%) was significantly higher than that of lymph node metastasis of cardioma adenocarcinoma with negative P53 immunoreactivity (29%, P<0.05). . In 37 cases of cardiac cancer tissues, 24 cases were negative for nm23 immunoreactivity, accounting for 65%. The lymph node metastasis rate (75%) in nm23-negative cases was significantly higher than that in nm23-positive cases (31%, P<0.05). In 10 cases of lymph node metastasis of cardiac cancer, the number of P53-positive cells in metastatic lymph nodes was significantly higher than that in the center. Tip: P53 and nm23 play a role in the metastasis of cardiac cancer.