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应用聚合酶链反应(PCR)扩增IgH重排产生的CDR-Ⅱ序列,检测23例急慢性B-淋巴细胞白血病患者。20例发生IgH重排,其中4例出现两种基因扩增产物。6例完全缓解的患者,3例检出异常重排的IgH基因,并先后复发。本文应用Southern blot法和PCR进行了对照性研究。
The CDR-Ⅱ sequences generated by IgH rearrangement were amplified by polymerase chain reaction (PCR), and 23 patients with acute and chronic B-lymphoblastic leukemia were detected. IgH rearrangement occurred in 20 cases, of which 4 cases appeared two kinds of gene amplification products. Six patients with complete remission, three cases of abnormal rearrangement detected IgH gene, and one after another. In this paper, the use of Southern blot and PCR control study.