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目的:建立一种快速准确检测大鼠肝微粒体中白花前胡甲素(Pd-Ia)含量的方法。方法:以华蟾毒精为内标物,采用Hypersil-C18(250mm×4.6mm,5μm)色谱柱,以甲醇-水(75∶25)为流动相,流速1mL·min-1,检测波长321nm,柱温25℃,进样量20μL。结果:Pd-Ia浓度在1～100μg·mL-1范围内与峰面积呈现良好的线性关系(r=0.9989);日内精密度RSD≤2.9%;日间精密度RSD≤8.5%;平均加样回收率(n=9)为98.4%。结论:本文建立的方法操作简单,可用于Pd-Ia含量的测定。
Objective: To establish a rapid and accurate method for the determination of Pd-Ia in rat liver microsomes. METHODS: Huasheng toxin was used as internal standard and Hypersil-C18 (250mm×4.6mm, 5μm) column was used. The mobile phase consisted of methanol-water (75∶25), the flow rate was 1mL·min-1, and detection wavelength was 321nm. Column temperature 25 °C, injection volume 20μL. Results: There was a good linear relationship between peak area and Pd-Ia concentration in the range of 1-100 μg·mL-1 (r=0.9989); intraday precision RSD ≤ 2.9%; inter-day precision RSD ≤ 8.5%; average loading The recovery rate (n=9) was 98.4%. Conclusion: The method established in this paper is simple and can be used for the determination of Pd-Ia content.