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目的 探讨人类白细胞抗原 (HLA) DRB1等位基因与胃腺癌及其临床特征和幽门螺杆菌(Hp)感染的关联性。方法 运用序列特异性引物聚合酶链反应和等位基因序列分析技术 ,检测无亲缘关系湖北省汉族健康人 136例、胃癌组 6 3例的HLA DRB1基因。内镜活检、Giemsa染色和 (或 )外周血ELISA检查胃黏膜Hp感染情况。SAS软件数据处理。 结果 HLA DRB10 90 1、12等位基因均与湖北省汉族人胃腺癌呈正相关 ;HLA DRB115等位基因则呈负相关。携带及非携带上述各等位基因患者 ,分别就其临床特征 ,包括平均患病年龄、性别比、肿瘤原发部位、TNM分期、肿瘤细胞分化程度以及Hp感染率等情况比较 ,差异均无显著性。结论 HLA DRB10 90 1、12等位基因均与湖北省汉族人胃腺癌呈正相关 ,而HLA DRB115则呈负相关。上述各相关的HLA DRB1等位基因与胃腺癌患者患病年龄、性别、TNM分期、肿瘤细胞分化程度无关 ;Hp感染虽为胃癌重要的致病因素 ,但并不是HLA DRB1等位基因通过增加Hp感染危险性 ,而影响胃腺癌的遗传易感性。
Objective To investigate the association of human leukocyte antigen (HLA) DRB1 alleles with gastric adenocarcinoma, its clinical features and Helicobacter pylori (Hp) infection. Methods Sequence-specific primer polymerase chain reaction and allele sequence analysis were used to detect HLA DRB1 gene in 136 unrelated healthy Han Chinese and 63 gastric cancer patients. Endoscopic biopsy, Giemsa staining, and (or) peripheral blood ELISA were used to detect Hp infection in gastric mucosa. SAS software data processing. Results The HLA DRB10 90 1 and 12 alleles were positively correlated with gastric adenocarcinoma in Hubei Han population. The HLA DRB115 allele was negatively correlated. The patients with and without the above-mentioned alleles had no significant differences in their clinical characteristics, including the average age of disease, sex ratio, primary tumor site, TNM stage, tumor cell differentiation, and Hp infection rate. Sex. Conclusion The HLA DRB10 90 1 and 12 alleles were positively correlated with gastric adenocarcinoma in Hubei Han population, while HLA DRB115 was negatively correlated. The above-mentioned related HLA DRB1 alleles were not associated with the age, sex, TNM stage, and tumor cell differentiation of patients with gastric adenocarcinoma; although Hp infection is an important pathogenic factor for gastric cancer, it is not that HLA DRB1 allele increases Hp. The risk of infection affects the genetic susceptibility of gastric adenocarcinoma.