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目的掌握广西壮族自治区(广西)口岸小兽自然感染汉坦病毒、立克次体、伯氏疏螺旋体、巴尔通体和钩端螺旋体(钩体)的情况。方法于2014-2015年在广西8个口岸辖区范围内采用鼠笼法捕获小兽,取其肺、肝和肾脏组织。从小兽肺中提取RNA,利用RT-PCR扩增汉坦病毒核酸;从小兽肝和肾脏中提取DNA,用PCR方法分别扩增立克次体、伯氏疏螺旋体、巴尔通体和钩体核酸。结果捕获黄胸鼠、褐家鼠和臭鼩鼱共86只,其中褐家鼠数量最多,占捕获总数的67.4%(58/86);汉坦病毒核酸、巴尔通体和钩体总阳性率分别为3.5%(3/86)、2.3%(2/86)和35.3%(6/17);未检测到立克次体和伯氏疏螺旋体核酸。结论广西地区部分口岸小兽可能有汉坦病毒、巴尔通体和钩体自然感染,应加强口岸小兽及其传播疾病的监测。
Objective To understand the natural infection of Hantavirus, rickettsiae, Borrelia burgdorferi, Bartonella and Leptospira (Leptospira) in small mammals at the port of Guangxi Zhuang Autonomous Region (Guangxi). Methods In 2014-2015, squirrel-cage method was used to capture small mammals in the area of Guangxi’s eight ports, taking the lung, liver and kidney tissues. RNA was extracted from small mammalian lungs, and the Hantaan virus was amplified by RT-PCR. DNA was extracted from small mammalian liver and kidney, and Rickettsiae, Borrelia burgdorferi, Bartonella and Leptospira were amplified by PCR. RESULTS: A total of 86 Rattus flavipectum, Rattus norvegicus and Stinky ragweed were captured, of which Rattus norvegicus was the largest, accounting for 67.4% (58/86) of the total trapping. The total positive rate of Hantaan virus nucleic acid, Bartonella, Were 3.5% (3/86), 2.3% (2/86) and 35.3% (6/17); no Rickettsia and Borrelia burgdorferi nucleic acids were detected. Conclusion Some animals in port may have natural infection with Hantavirus, Bartonella and Leptospira, and the monitoring of small mammals and their transmitted diseases should be strengthened.