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为研究用细胞内柔红霉素（ＤＮＲ）的荧光直方图诊断白血病耐药的方法，用流式细胞仪（ＦＣＭ）检测了２０例急性髓性白血病（ＡＭＬ）的荧光直方图，结合患者的临床耐药表现，耐药细胞的荧光强度诊断界点，耐药指数和荧光指数进行分析。结果，初治４例诊断为药敏，１例诊断为原发耐药；１５例有耐药临床表现的病例，１２例诊断为经典耐药，３例诊断为再生耐药。该方法可快速、简便、直观地在临床上及时发现和评价白血病耐药的类型和程度，为指导临床制定个体化的化疗方案提供依据
To investigate the method of diagnosing drug resistance of leukemia with fluorescence histogram of intracellular daunorubicin (DNR), fluorescence histograms of 20 patients with acute myeloid leukemia (AML) were detected by flow cytometry (FCM), combined with The clinical manifestations of drug resistance, the diagnostic points of fluorescence intensity of drug resistant cells, the drug resistance index and the fluorescence index were analyzed. Results, initially diagnosed as drug sensitivity in 4 cases, 1 case of diagnosis of primary drug resistance; 15 cases of clinical manifestations of drug resistance, 12 cases were diagnosed with classical drug resistance, 3 cases were diagnosed with relapse drug resistance. The method can quickly, easily and intuitively detect and evaluate the type and degree of leukemia drug resistance in a timely manner in clinical practice, and provide a basis for guiding the clinical development of individualized chemotherapy regimens.