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目的探讨酪氨酸激酶c-kit蛋白的表达及血清干细胞因子(stem cell factor,SCF)水平在大鼠化疗后痞满证发病机制中的作用及消痞方干预对其的影响。方法将60只Wistar大鼠随机分为空白组、模型组、西沙必利组、消痞方高剂量组、消痞方中剂量组、消痞方低剂量组,每组10只。除空白组外,其余5组采用尾静脉注射化疗药物建立大鼠脾虚证模型。空白组和模型组给予0.9%Na Cl溶液灌胃,消痞方低剂量组、中剂量组与高剂量组分别给予浓度100%、150%、200%的煎剂灌胃,西沙必利组给予西沙必利灌胃,共给药7天。观察各组大鼠的一般情况,采用酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)法检测大鼠血清SCF水平,采用免疫组化方法检测大鼠胃肌层c-kit蛋白表达水平。结果模型组大鼠胃肌层c-kit蛋白表达及血清SCF水平明显低于空白组(P<0.05)。消痞方高剂量组与模型组比较,胃肌层c-kit蛋白表达及血清SCF水平显著升高(P<0.05),且明显优于西药组。消痞方低剂量及中剂量组较模型组各指标差异无统计学意义(P>0.05)。结论消痞方能够提高大鼠胃肌层c-kit蛋白的表达及血清SCF水平,维持c-kit/SCF信号通路的稳定,恢复胃肠道运动功能,从而起到对化疗所致痞满的治疗作用。
Objective To investigate the role of tyrosine kinase c-kit protein expression and the level of serum stem cell factor (SCF) in the pathogenesis of post-chemotherapy chemotherapy for malignant pleural effusion in rats and the effect of Xiaopi prescription on it. Methods Sixty Wistar rats were randomly divided into blank group, model group, cisapride group, Xiaopi Fang high dose group, Xiaopu Fang middle dose group and Xiaopi Fang low dose group, with 10 rats in each group. In addition to the blank group, the remaining 5 groups were injected splenic vein with chemotherapy drugs to establish the model of spleen deficiency in rats. The rats in the blank group and the model group were given gavage with 0.9% NaCl solution. The Xiaopi Fang low-dose group, the middle-dose group and the high-dose group were orally administered with decoction of 100%, 150% and 200% respectively. The cisapride group Cisapride intragastric administration, a total of 7 days. The general situation of rats in each group was observed. Serum SCF level was measured by enzyme-linked immunosorbent assay (ELISA), and the expression of c-kit protein was detected by immunohistochemistry. Results The expression of c-kit protein and the level of serum SCF in gastric myocytes in model group were significantly lower than those in blank group (P <0.05). Compared with the model group, the expression of c-kit protein and the level of serum SCF in the high-dose Xiaopi Fang group were significantly increased (P <0.05), and were significantly better than those in the western medicine group. Xiaopi Fang low dose and middle dose group compared with the model group, there was no significant difference (P> 0.05). Conclusion Xiaopi prescription can improve the expression of c-kit protein and serum level of SCF in gastric myocytes, maintain the stability of c-kit / SCF signaling pathway and restore the function of gastrointestinal motility, and thus play a role in chemotherapy-induced fullness of the liver Therapeutic effect.