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目的探讨接种疫苗诱导免疫应答的机制,为疫苗的研发和应用提供理论基础。方法运用整合分析(Meta分析),分析自美国生物技术信息中心(National Center for Biotechnology Information,NCBI)的基因表达数据库(Gene Expression Omnibus,GEO)中下载的3套疫苗免疫基因芯片表达谱数据,初步筛选出疫苗免疫机体后获得性免疫在转录水平上的关键基因及通路。结果应用Meta分析得到疫苗免疫3套数据集的共同差异基因1 403个,其中表达水平上调基因709个,主要包括人类真核翻译延伸因子(EEF1A1)、白细胞介素18(IL-18)和核糖体蛋白大亚基P2(RPLP2)等,富集出自然杀伤细胞(natural killer,NK)介导的细胞毒作用和B细胞受体信号通路等26条通路;表达水平下调的基因694个,主要包括核糖体蛋白L41(RPL41)、核糖体蛋白L32(RPL32)、核糖体蛋白L34(RPL34)和胞浆多聚A结合蛋白3(PABPC3)等,富集出氧化磷酸化和T细胞受体信号通路等11条通路。结论应用Meta分析方法获得多个疫苗免疫机体后诱导获得性免疫的关键基因及通路,对了解疫苗的免疫机制具有重要意义。
Objective To explore the mechanism of vaccine-induced immune response and provide a theoretical basis for the development and application of the vaccine. Methods Using integration analysis (Meta-analysis), we analyzed expression profiles of three sets of vaccine-immunized gene chips downloaded from the Gene Expression Omnibus (GEO) database of the National Center for Biotechnology Information (NCBI) The key genes and pathways at the transcriptional level of adaptive immunity after vaccination were screened out. Results A total of 1 403 genes were found to be differentially expressed in 3 sets of data sets of the three sets of vaccines, including 709 up-regulated genes, including EEF1A1, IL-18 and ribose Large subunit P2 (RPLP2), which is rich in natural killer (NK) -mediated cytotoxicity and B cell receptor signaling pathways such as 26 pathways; down-regulated genes 694, the main Including ribosomal protein L41 (RPL41), ribosomal protein L32 (RPL32), ribosomal protein L34 (RPL34), and cytoplasmic poly A binding protein 3 (PABPC3), are enriched in oxidative phosphorylation and T cell receptor signaling Pathways and other 11 routes. Conclusions The key genes and pathways for acquiring adaptive immunity after multiple vaccines are immunized are obtained by Meta analysis. It is of great significance to understand the immunological mechanism of vaccines.