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本文利用35对SSR引物对23份水稻品种(组合)进行遗传多样性分析。结果表明,35对引物在23个水稻品种上共获得342条扩增条带,其中多态性条带288条,多态性条带所占比例为84.21%。23个参试水稻品种对稻曲病的抗性表现出明显差异,病穗率范围为8.20%~75.93%,最高和最低病穗率之间相差67.73个百分点,病情指数范围为2.19~52.67,最高和最低病情指数相差50.48。成对品种的GS变化为0.614~0.954,平均值为0.740单个品种的平均GS值变化为0.686~0.778之间。共有11份供试品种的平均GS值低于GS总平均值(0.740),其它12份(占供试品种总数的52.2%)供试品种平均GS值高于GS总平均值(0.740)。聚类分析的结果表明,在遗传相似系数为0.75时,23个供试品种可分为5类。不同生育期的品种可以聚在同一类群中,聚在同一类的品种生育期最多可相差11 d,而同一生育期的品种可以分属不同的类,包括中抗到高感的不同抗性类型。
In this study, 35 pairs of SSR primers were used to analyze the genetic diversity of 23 rice cultivars (combinations). The results showed that 342 pairs of amplified bands were obtained from 35 pairs of primers on 23 rice cultivars, of which 288 were polymorphic bands and 84.21% were polymorphic bands. The resistance of 23 tested rice cultivars to False smut was significantly different, ranging from 8.20% to 75.93% for panicle disease and 67.73% for the highest and lowest panicle disease. The disease index ranged from 2.19 to 52.67, The difference between the highest and lowest disease index was 50.48. The GS of the pair varieties varied from 0.614 to 0.954 with an average of 0.740. The average GS value of single varieties varied from 0.686 to 0.778. The average GS value of 11 tested cultivars was lower than the average value of GS (0.740), while the average of other 12 cultivars (52.2% of the total cultivars tested) was higher than the average of GS (0.740). The results of cluster analysis showed that at the genetic similarity coefficient of 0.75, 23 tested cultivars could be divided into 5 categories. Varieties at different growth stages can be clustered in the same group, with the maximum difference of 11 d in the same type of cultivars, while the varieties in the same growth period can belong to different classes, including different resistant types with medium to high susceptibility .