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用DMDcDNA8探针从Dystrophin基因的YAC克隆的Cosmid亚克隆库中筛选到含Dystrophin基因的第51号外显子Cosmid克隆,将该Cosmid亚克隆于pUC118中,获得合Dystrophin基因第51导外星子的3.1kb—HindⅢ片段的亚克隆,该片段的长度为3179bp,含Dystrophin基因第家51号外星子的全部和第50和第51导内含子的部分,对第50和51号内含子的核苷酿顺序分析表明,在这两个内台子中共发现36个短段串联重复顺序.22正向和反向重复顺序和8个同原顺序。提出重复顺序之间的重组可能导致第51号外显子缺失的假设。
The Cosmid clone of exon 51 containing the Dystrophin gene was screened from the YAC cloned Cosmid subclone library of the Dystrophin gene using the DMD cDNA 8 probe. The Cosmid subclone was subcloned into pUC118 to obtain 3.1 of the Dystrophin gene, The subcloning of the kb-HindIII fragment, which was 3179 bp in length, contained all and 50 and 51 intron parts of the Dystrophin gene family No. 51, The analysis of brewing sequence showed that a total of 36 short tandem repeats were found in the two internal sets. 22 forward and reverse repeat order and 8 same order. The hypothesis that recombination between repeats may result in the deletion of exon 51 is proposed.