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目的:探讨不同剂量瘦素预处理对糖尿病大鼠心肌缺血再灌注损伤的保护作用。方法:将72只大鼠随机分为对照组、假手术组、心肌梗死组以及大、中、小剂量瘦素预处理组。对照组给予普通饲料喂养,其余各组均给予高糖高脂喂养。1个月后,采大鼠静脉血,检测其血清FBG、FTNS、瘦素、HOMA、TNF-α、MDA及血脂水平。结果:与对照组相比,其余各组FBG、FINS、瘦素水平及HOMA-IR均明显升高(P<0.05);心肌梗死组及大、中、小剂量瘦素预处理组FBG及瘦素水平与假手术组相比均明显升高,心肌梗死组、高剂量瘦素预处理组FINS及HOMA-IR与之相比亦明显升高,小、中剂量瘦素预处理组FINS及HOMA-IR与之相比均明显降低(P<0.05);小、中剂量瘦素预处理组FBG、FINS、瘦素水平及HOMA-IR与心肌梗死组相比均明显降低(P<0.05)。与对照组相比,其余各组大鼠TNF-α、MDA水平均明显升高(P<0.05);与假手术组相比,心肌梗死组、大、中、小剂量组大鼠TNF-α、MDA水平明显升高(P<0.05);与心肌梗死组相比,中、小剂量组大鼠TNF-α、MDA水平均明显降低(P<0.05)。与对照组相比,其余各组大鼠TG、TC、LDL-C水平均明显升高,HDL-C水平均明显降低(P<0.05);大、中、小剂量组HDL-C值与假手术组相比均明显升高,TG、TC、LDL-C值与之相比均明显降低(P<0.05);与心肌梗死组相比,小、中剂量组大鼠TG、TC、LDL-C水平均明显降低(P<0.05),HDL-C水平均明显升高(P<0.05)。结论:瘦素预处理能够保护糖尿病大鼠心肌缺血再灌注损伤,可能与其减轻炎症、氧化应激、血脂紊乱及胰岛素抵抗有关。
Objective: To investigate the protective effects of different doses of leptin on myocardial ischemia-reperfusion injury in diabetic rats. Methods: Seventy two rats were randomly divided into control group, sham operation group, myocardial infarction group, and large, medium and low dose leptin pretreatment groups. The control group was fed with normal feed, while the remaining groups were given high-sugar and high-fat diet. One month later, the venous blood of rats was collected and the serum levels of FBG, FTNS, leptin, HOMA, TNF-α, MDA and blood lipid were measured. Results: FBG, FINS, leptin levels and HOMA-IR of the other groups were significantly higher than those of the control group (P <0.05). FBG and leptin in the myocardial infarction group and the leptin pretreatment group The levels of FINS and HOMA-IR in myocardial infarction group and high-dose leptin pretreatment group were also significantly higher than those in sham operation group. FINS and HOMA in small and medium dose leptin pretreatment group (P <0.05). The levels of FBG, FINS, leptin and HOMA-IR in small and medium dose leptin pretreatment group were significantly lower than those in myocardial infarction group (P <0.05). Compared with the control group, the levels of TNF-α and MDA in the other groups were significantly increased (P <0.05). Compared with the sham operation group, the levels of TNF-α (P <0.05). Compared with myocardial infarction group, the levels of TNF-α and MDA in middle and small dose groups were significantly decreased (P <0.05). Compared with the control group, the levels of TG, TC and LDL-C in the other groups were significantly increased, and the HDL-C levels were significantly decreased (P <0.05) TG, TC and LDL-C were significantly lower than those in the operation group (P <0.05). Compared with the myocardial infarction group, TG, TC, LDL- C levels were significantly lower (P <0.05), HDL-C levels were significantly increased (P <0.05). Conclusion: Leptin preconditioning can protect myocardium against ischemia-reperfusion injury in diabetic rats and may be related to the reduction of inflammation, oxidative stress, dyslipidemia and insulin resistance.