论文部分内容阅读
目的探讨辛伐他汀是否依赖核因子(NF)-κB信号通路分子的变化诱导K562细胞凋亡。方法体外培养K562细胞,首先用流式细胞术检测辛伐他汀对K562细胞凋亡的影响,RT-PCR分析NF-κB信号通分子转录水平的变化。然后构建裸鼠K562细胞移植瘤模型,TUNEL法检测辛伐他汀对裸鼠移植的K562细胞晚期凋亡的影响,RT-PCR检测裸鼠体内K562细胞NF-κB信号通路分子转录水平的变化。结果辛伐他汀在体外能明显引起K562细胞发生凋亡,NF-κB通路大多数分子出现差异表达。不同剂量的辛伐他汀能够诱导裸鼠身上移植的K562细胞发生凋亡,并随药物剂量的增加凋亡率逐渐增高(P<0.01);辛伐他汀能够引起NF-κB信号通分子在转录水平出现明显差异表达(P<0.01)。结论辛伐他汀可能通过引起NF-κB信号通路分子的变化来诱导K562细胞凋亡的发生。
Objective To investigate whether simvastatin can induce the apoptosis of K562 cells by the changes of nuclear factor (NF) -κB signaling molecules. Methods K562 cells were cultured in vitro. Firstly, the effect of simvastatin on the apoptosis of K562 cells was detected by flow cytometry. The transcription level of NF-κB signal transduction pathway was analyzed by RT-PCR. Then the nude mice model of K562 cell xenograft was established. TUNEL assay was used to determine the effect of simvastatin on the late apoptosis of K562 cells transplanted in nude mice. The transcription level of NF-κB signal transduction pathway in K562 cells was detected by RT-PCR. Results Simvastatin could obviously induce the apoptosis of K562 cells in vitro, and most of the molecules in NF-κB pathway were differentially expressed. Different doses of simvastatin could induce the apoptosis of K562 cells transplanted in nude mice, and the apoptotic rate increased gradually with the increase of the dosage of simvastatin (P <0.01). Simvastatin can induce the transcription of NF-κB signaling Significantly different expression (P <0.01). Conclusion Simvastatin may induce the apoptosis of K562 cells by inducing the changes of NF-κB signaling pathway.