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目的·观察半胱氨酸组织蛋白酶S(CatS)基因敲除对高岭土诱导小鼠交通性脑积水严重程度的影响。方法·针对8只CatS基因敲除(CatS~(-/-))小鼠和16只野生型(WT)C57BL/6小鼠,通过枕大池注射高岭土混悬液的方法建立交通性脑积水模型,建模前及建模后1周利用头颅磁共振(MRI)扫描测量小鼠侧脑室体积并计算侧脑室指数,比较2组小鼠脑积水严重程度。结果·建模后1周CatS~(-/-)组死亡1只(12.5%),WT组死亡2只(12.5%),2组间死亡率的差异无统计学意义(P=1.000);头颅MRI结果显示存活CatS~(-/-)组和WT组小鼠均有不同程度脑室扩大,建模前CatS~(-/-)组(n=8)侧脑室指数为0.05±0.01,WT组(n=16)侧脑室指数为0.04±0.01(P=0.720)。建模后1周CatS~(-/-)组(n=7)侧脑室指数为0.13±0.02,其中侧脑室指数增加1倍以上者5只,占71.4%;WT组(n=14)侧脑室指数为0.11±0.01,侧脑室指数增加1倍以上者10只,占71.4%;2组比较,差异无统计学意义(P=0.950)。结论·枕大池注射高岭土混悬液后1周,小鼠侧脑室明显扩大伴脑积水发生,模型成功率高;CatS基因敲除对高岭土诱导小鼠脑交通性脑积水发展无明显影响。
Objective · To observe the effect of cysteine cathepsin S (CatS) knockout on the severity of traumatic hydrocephalus induced by kaolin in mice. METHODS · Traffic-induced hydrocephalus was established by injection of a kaolin suspension into eight CatS knockout (CatS ~ (- / -)) mice and 16 wild type (WT) C57BL / 6 mice One week before modeling and one month after modeling, the volume of lateral ventricle of mice was measured by MRI and the index of lateral ventricle was calculated. The severity of hydrocephalus was compared between the two groups. Results One death (12.5%) in Cats ~ (- / -) group and two deaths in WT group (12.5%) at one week after modeling were not statistically significant (P = 1.000). The results of cranial MRI showed that ventricle enlargement was found in Cats ~ (- / -) and WT groups, and the index of lateral ventricle in Cats ~ (- / -) group before modeling was 0.05 ± 0.01 Group (n = 16) lateral ventricle index was 0.04 ± 0.01 (P = 0.720). One week after modeling, the index of lateral ventricle in Cats ~ (- / -) group (n = 7) was 0.13 ± 0.02, in which the index of lateral ventricle increased more than 1 time, accounting for 71.4% Ventricular index was 0.11 ± 0.01, lateral ventricle index increased more than 1 times 10, accounting for 71.4%; 2 groups, the difference was not statistically significant (P = 0.950). Conclusions · One week after injection of kaolin suspension in occipital large pool, the lateral ventricles of mice significantly expand with hydrocephalus, and the model success rate is high. CatS gene knockout has no obvious effect on the development of hydrocephalus in mice induced by kaolin.