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目的 分析2009-2011年广州地区甲型H1N1流感病毒对奥司他韦的耐药情况.方法 选取2009年7月至2011年4月从广州地区分离的甲型H1N1流感病毒192株,通过对病毒的血凝素和神经氨酸酶基因进行序列测定,并利用数据库和软件进行生物信息学分析；测定病毒对奥司他韦的敏感度,分析广州地区甲型H1N1流感病毒的耐药情况和变异规律.结果 192株中1株神经氨酸酶基因发生S247N神经氨酸酶耐药突变,对奥司他韦的敏感活性(IC)50为0.45 nmol/L,比野生株下降了2.5倍.进化分析结果表明该耐药株未在广州地区流行,且与2011年初澳大利亚和新加坡流行的S247N耐药株不处于同一进化分支.结论 广州地区甲型H1N1流感病毒对奥司他韦耐药的比例低,但需要加强耐药株的监测,防控来白境外输入性耐药株的传播.“,”Objective To study the prevalence of osehamivir-resistance among pandemic A (H1N1)2009 viruses isolated from patients in Guangzhou between 2009 and 2011,and to provide more information for clinical usage of osehamivir.Methods Totally 192 pandemic A (H1N1)2009 viruses isolated from patients in Guangzhou between July 2009 and April 2011 were studies.The HA and NA genes of all strains were sequenced to reveal the evolution of viruses,and the susceptibility of viruses to osehamivir was tested in vitro.Results One strain with a S247N mutation of the NA gene,which would make the virus resistant to oseltamivir,was found.The susceptibility (IC)50 of this viral strain to osehamivir was 0.45 nmol/L,2.5 times lower as compared to the wild-type strains.Phylogenetic analysis showed that this virus was not prevalent in Guangzhou from 2009-2011,and was not located in the same branch with the strains being epidemic in Australia and Singapore during the early seasons of 2011.Conclusion The resistance rate of pandemic A(H1 N1)2009 viruses isolated from Guangzhou to oseltamivir was low,but surveillance on resistant strains needs to be strengthened to control resistant viruses imported from abroad.