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目的：观察特女贞甙（ＳＰＮ）体外对小鼠脾淋巴细胞转化和自然杀伤细胞活性的影响。方法：分别采用（１）３Ｈ－ＴｄＲ掺入法，以核素掺入量为测定指标；（２）３Ｈ－ＴｄＲ标记靶细胞释放法，以杀伤率和抑制率为测定指标。结果：ＳＰＮ能与亚适量刀豆球蛋白Ａ（ＣｏｎＡ）协同提高正常鼠脾Ｔ淋巴细胞的转化；ＳＰＮ对正常鼠脾自然杀伤细胞（ＮＫ）活性无明显作用（Ｐ＞０．０５），但能显著提高由环磷酰胺抑制的ＮＫ杀伤功能（Ｐ＜０．０１），减轻由地塞米松引起的ＮＫ活性低下作用（Ｐ＜０．０１）。结论：ＳＰＮ为中药女贞子中具有免疫活性的水溶性成分之一。
Objective: To observe the effect of SPN on the spleen lymphocyte transformation and natural killer cell activity in mice. METHODS: (1) 3H-TdR incorporation method was used to determine the incorporation of radionuclides. (2) 3H-TdR labeled target cell release method was used to determine the killing rate and inhibition rate. RESULTS: SPN could synergistically increase the conversion of spleen T lymphocytes in normal mice with subcontractor ConA, and SPN had no significant effect on the natural killer cell (NK) activity of normal mice (P>0.05). Can significantly improve the inhibition of NK killing by cyclophosphamide (P <0.01), reduce the dexamethasone-induced NK activity (P <0.01). Conclusion: SPN is one of the water-soluble components with immunological activity in Ligustrum lucidum.