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目的:研究基质细胞衍生因子-1α(stromal cell derived-factor-1α,SDF-1α)及其受体CXCR7在介导骨髓间充质干细胞(bone marrow derived mesenchymal stem cells,BMSCs)向大鼠脑缺血区迁移的作用。方法:BMSCs的原代培养、四血管阻断脑缺血模型制备大鼠脑缺血模型、侧脑室移植组(培养基干预组、BMSCs组和CXCR7抗体预处理BMSCs组)、免疫组织化学和免疫荧光组织化学染色方法。结果:免疫组织化学染色结果显示:在各组大鼠海马CA1区细胞内可见不同程度的呈黄色或棕黄色颗粒的SDF-1α免疫阳性产物,主要表达在细胞胞质中,CXCR7蛋白则主要表达在胞核和胞膜中;与假手术组比较,BMSCs组和CXCR7抗体预处理BMSCs组SDF-1α和CXCR7的表达均升高(P<0.05)。免疫荧光组织化学结果显示:侧脑室注射BMSCs并移植48 h后,BMSCs组、CXCR7抗体预处理BMSCs组海马CA1区均有不同程度荧光标记的阳性细胞;与BMSCs组比较,CXCR7抗体预处理BMSCs组阳性细胞数降低(P<0.05)。结论:缺血/再灌注损伤后,促进了SDF-1α及CXCR7阳性产物的表达;同时SDF-1α/CXCR7能够促进BMSCs向损伤区域的迁移。
OBJECTIVE: To investigate the effects of stromal cell derived-factor-1α (SDF-1α) and its receptor CXCR7 on the induction of bone marrow derived mesenchymal stem cells (BMSCs) The role of blood migration. Methods: The primary culture of BMSCs and the model of cerebral ischemia induced by four-vessel occlusion were used to establish the rat model of cerebral ischemia. The rats in the lateral ventricle transplantation group (medium intervention group, BMSCs group and CXCR7 antibody pretreatment BMSCs group), immunohistochemistry and immunology Fluorescence histochemical staining method. Results: The results of immunohistochemistry showed that SDF-1α immunopositive products with different levels of yellow or brown granules were observed in hippocampal CA1 area of rats in each group, mainly expressed in the cytoplasm of the cells, while CXCR7 protein was mainly expressed In the nucleus and membrane, compared with the sham group, the expression of SDF-1α and CXCR7 in BMSCs group and CXCR7 antibody-pretreated BMSCs group were significantly increased (P <0.05). Immunofluorescence histochemistry results showed that: after intracerebroventricular injection of BMSCs and transplantation for 48 h, BMSCs group and CXCR7 antibody-pretreated BMSCs group showed hippocampal CA1 region with different degrees of fluorescence-labeled positive cells; Compared with BMSCs group, CXCR7 antibody pretreated BMSCs group The number of positive cells decreased (P <0.05). CONCLUSION: The expression of SDF-1α and CXCR7-positive products is promoted after ischemia / reperfusion injury. SDF-1α / CXCR7 can promote the migration of BMSCs to injured area.