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目的:筛选能与炎症相关趋化因子结合的广谱抑制剂先导物。方法:用内毒素刺激外周血单核细胞建立急性炎症模型,以刺激炎症相关趋化因子的产生,利用噬菌体随机12肽库进行高通量筛选得到阳性克隆,并初步分析其体外活性。结果:经过四轮肽库亲和筛选后,获得了一共同短肽(TVVGSGCVLRIQ)。结论:该短肽可有效抑制炎性趋化因子活性,说明该短肽有可能成为一种广谱的炎性趋化因子抑制先导物。
OBJECTIVE: To screen broad-spectrum inhibitor leaders that bind to inflammatory-associated chemokines. Methods: Acute inflammation model was established by stimulating peripheral blood mononuclear cells with endotoxin to stimulate the production of inflammatory related chemokines. The positive clones were screened by phage random 12-peptide library and the activity in vitro was analyzed. Results: After four rounds of peptide library affinity screening, a common short peptide (TVVGSGCVLRIQ) was obtained. Conclusion: The short peptide can effectively inhibit the activity of inflammatory chemokines, indicating that the short peptide may become a broad spectrum of inflammatory chemokine inhibition lead.