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目的:用高效液相色谱法测定十味活血丸中橙皮苷的含量。方法:选用Diamonsil TM-C18分析柱(150 mm×4．6 mm, 5μm),以甲醇-水(36:64)为流动相,检测波长284 nm,流速为1．0 ml·min-1。结果:橙皮苷在0．41～2．86μg范围内,峰面积与进样量呈线性关系(r=0．999 7),橙皮苷中平均回收率为102．91％(RSD=1．84％)。结论:本法操作简便,结果可靠,重现性好,可作为十味活血丸质量控制的方法。
Objective: To determine the content of hesperidin in Shiwei Huoxue Pills by HPLC. Methods: The Diamonsil TM-C18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol-water (36:64). The detection wavelength was 284 nm and the flow rate was 1.0 ml · min-1. RESULTS: Hesperidin ranged from 0.41 to 2.86 μg, and the peak area was linear with the injection volume (r=0.999 7). The average recovery rate of hesperidin was 102.91% (RSD=1). .84%). Conclusion: This method is simple, reliable and reproducible. It can be used as a method for quality control of Shiwei Huoxue pills.