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目的探讨亚急性氟中毒小鼠抗氧化能力及肾功能的变化。方法实验时间为10d,将40只昆明种小鼠随机分对照组10只和中毒组30只,雌雄各半。对照组小鼠腹腔注射生理盐水13ml/(kg.d),中毒组小鼠腹腔注射氟化钠溶液(1.0g/L)13ml/(kg.d)。第10天剪尾取血测定血浆中谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量、尿素(Urea)含量和肌酐(Cr)含量。结果中毒组与对照组相比血浆MDA含量明显升高[(7.39±1.67)vs(5.89±0.81),t=3.52,P<0.01],GSH-Px活性和Urea含量、Cr含量三项指标比较,差异无统计学意义[(426.40±50.92)vs(422.0±52.24),(7.32±1.60)vs(6.87±2.14)]。结论本实验结果证明,亚急性氟中毒小鼠抗过氧化能力明显下降,尚未见到对肾排泄功能的严重影响。
Objective To investigate the changes of anti-oxidation and renal function in sub-acute fluorosis mice. Methods The experimental time was 10 days, and 40 Kunming mice were randomized into 10 mice in the control group and 30 mice in the poisoning group. Control mice were intraperitoneally injected with saline (13ml / (kg · d)), and poisoned mice were injected intraperitoneally with sodium fluoride solution (1.0g / L) at 13ml / (kg · d). On the 10th day, the blood samples were collected and tailored for determination of GSH-Px activity, malondialdehyde (MDA) content, urea content and creatinine (Cr) content. Results Compared with the control group, the content of MDA in the poisoning group was significantly increased (7.39 ± 1.67 vs 5.89 ± 0.81, t = 3.52, P <0.01). The GSH-Px activity, Urea content and Cr content in the poisoning group were significantly higher than those in the control group (426.40 ± 50.92) vs (422.0 ± 52.24), (7.32 ± 1.60) vs (6.87 ± 2.14), respectively. Conclusions The results of this experiment demonstrate that the anti-peroxidation ability of sub-acute fluorosis mice was significantly decreased and no serious effect on renal excretion function was observed.