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目的：研究急性心肌梗死（ＡＭＩ）时脂蛋白（ａ）〔ＬＰ（ａ）〕、血小板（ＰＬＴ）及蛋白激酶Ｃ（ＰＫＣ）的关系。方法：分别以高ＬＰ（ａ）血症的ＡＭＩ患者（３２例）及健康对照者（３２ 例）为对象，采用全自动血细胞分析仪检测其ＰＬＴ数量，双抗夹心ＥＬＩＳＡ单克隆抗体法测定ＬＰ（ａ）及液闪仪测定ＰＬＴ中ＰＫＣ底物４０ ｋｕ 蛋白磷酸化程度，对检测结果进行统计学分析。结果：ＡＭＩ组ＰＬＴ数目及４０ ｋｕ 蛋白磷酸化程度明显高于对照组（Ｐ＜ ０．０５），且磷酸化程度随着４０ ｋｕ 蛋白磷酸化反应时间的延长而增强（ Ｐ＜ ０．０５）。结论：ＬＰ（ａ）是ＡＭＩ的独立危险因素。高ＬＰ（ａ）血症的ＡＭＩ患者ＰＬＴ数目及ＰＬＴ中ＰＫＣ底物４０ ｋｕ 蛋白磷酸化程度均增高
Objective: To investigate the relationship between lipoprotein (a) [LP (a)], platelet (PLT) and protein kinase C (PKC) in acute myocardial infarction (AMI) Methods: The patients with AMI (32 cases) and healthy controls (32 cases) with high LP (a) hyperlipidemia were enrolled in this study. The number of PLT was detected by automatic hematology analyzer. The level of PLT was determined by double-antibody sandwich ELISA (A) and the determination of the phospholipid level of PKC substrate 40 ku protein in PLT by liquid scintillation counting, the results were statistically analyzed. Results: The number of PLT and phosphorylation of 40 ku in AMI group were significantly higher than those in control group (P <0.05), and the phosphorylation level increased with the increase of 40 ku phosphorylation reaction time (P <0.05) . Conclusion: LP (a) is an independent risk factor for AMI. The number of PLTs in patients with high LP (a) hyperlipidemia and the phosphorylation of 40 ku protein of PKC substrate in PLT increased