Oleanolic acid-induced apoptosis and its relation with intracellular calcium in human lung adenocarc

• 作 者: Xiao-Hong Wei Ai-Qun Ma Jie Sh
• 机 构: Department of Respiratory Medicine,Department of Cardiovascular Disease,Department of Medicine
• 出 处: 西安交通大学学报（英文版）
• 发表日期: 2004年期
• 关键词: oleanolic acid A549 cell apoptosis intracellular calcium flow cytometry

Objective To investigate the effect of oleanolic acid (OA) on apoptosis, correlation between apoptosis and intracellular calcium, and its mechanism in human lung adenocarcinoma cell line A549. Methods Human lung adenocarcinoma A549 cells were incubated in vitro and assigned with OA concentrations of 0, 10, 20 and 40μg/mL. The apoptosis status of A549 cell line was detected with Annexin V-FITC/PI by flow cytometry (FCM); fluorescence intensity (FI) of A549 cells was assessed and the level of intracellular calcium was calculated at 24 hour of OA intervention. The relation between apoptosis and calcium FI was illustrated by curve fitting. Results FCM showed that 10, 20 and 40μg/mL of OA could induce A549 cell apoptosis, which followed a concentration-effect pattern; 24-hour intervention with 20μg/mL and 40μg/mL OA showed increased A549 cell apoptosis, and was significantly different from that with 0μg/mL OA (P