论文部分内容阅读
目的：研究高效液相色谱法测定石杉碱甲的方法及条件。方法：色谱柱ＡｌｔｉｍａＣ１８，流动相为甲醇－０．１ｍｏｌ·Ｌ－１醋酸铵缓冲液（ｐＨ６．０）３６∶６４，检测波长３０８ｎｍ。结果：石杉碱甲在３．０～７．０μｇ·ｍｌ－１范围内线性关系良好，其回归方程为Ｙ＝１８１１．８７Ｘ－２３４．９０，ｒ＝０．９９９９，平均回收率为９９．９％。结论：本法快速，准确，重现性好
Objective: To study the method and conditions for determination of Huperzine A by high performance liquid chromatography. Methods: Altima C18 column was used. The mobile phase consisted of methanol-0.1 mol·L-1 ammonium acetate buffer (pH 6.0) at 36:64 and the detection wavelength was at 308 nm. Results: Huperzine A had a good linearity in the range of 3.0-7.0 μg · ml-1 with a regression equation of Y = 1811.87X-234.90 and r = 0.9999 with an average recovery of 99%. 9%. Conclusion: This method is fast, accurate and reproducible