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目的通过在不同温度条件下对霍乱弧菌生存能力的观察,探索大连市水域中霍乱弧菌在流行“间歇期”如何存活和越冬。方法在自然海水和湖水中分别接种本市分离出的埃尔托型霍乱弧菌流行株小川1b型(大E940014)和埃尔托型霍乱弧菌非流行株小川32l(大E940011),在1～2℃、5～6℃及10～12℃3个温度条件下检测霍乱弧菌生存能力。结果流行株(小川1b型)在1～2℃海水存活26 d,5～6℃存活28 d,10～12℃存活46 d。可见,10～12℃生存时间明显高于1～2℃或5～6℃(P<0.05)。结论两类菌株的生存对环境温度均很敏感,温度越低死亡越快。在自然水体中,非流行株生存时间略长于流行株;而在除菌水体中两类菌存活能力基本一致。
Objective To investigate the survival of Vibrio cholerae in different temperature conditions and to find out how Vibrio cholerae in Dalian waters survive and pop in the epidemic “intermittent period”. Methods In natural seawater and lake water, the Elbow-type Vibrio cholerae strains Ogawa 1b (E940014) and Olle-type non-endemic strains of Vibrio cholerae Ogawa 32l (E940011) ~ 2 ℃, 5 ~ 6 ℃ and 10 ~ 12 ℃ three temperature conditions Vibrio cholerae viability. Results The epidemic strain (Ogawa 1b) survived seawater at 1 ~ 2 ℃ for 26 days, survived at 5 ~ 6 ℃ for 28 days and survived at 10 ~ 12 ℃ for 46 days. Visible, 10 ~ 12 ℃ survival time was significantly higher than 1 ~ 2 ℃ or 5 ~ 6 ℃ (P <0.05). Conclusion The survival of the two strains is very sensitive to the ambient temperature. The lower the temperature, the faster the death. In natural waters, the survival time of non-epidemic strains was slightly longer than that of the epidemic strains, while the survival ability of the two kinds of bacteria in the bacteria-removing water bodies was basically the same.