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目的研究福州地区2004年无菌性脑膜炎病原——埃柯病毒30型(ECHO30)VP1区基因特征及其分子流行病学特点。方法选取福州地区2004年2株ECHO30(FJ-C2-04、FJ-C7-04),进行核糖核酸(RNA)提取和VP1区逆转录-聚合酶链反应(RT-PCR),测定和分析PCR产物的核苷酸序列。结果所测2株ECHO30VP1区基因全长都是876bp,翻译的氨基酸全长292aa。2株病毒VP1区核苷酸同源性为99.89%(仅相差1个bp),氨基酸序列完全相同。FJ-C2-04株VP1区基因全长和基因库(GenBank)上检索的相关ECHO30毒株比较,同源性为75.66%～97.49%。构建的ECHO30遗传进化树将已知的ECHO30划分为7个组,福州地区分离的ECHO30在第7组。结论ECHO30随着年代沿革不断衍化,福州地区分离株与早期分离株差异最大,与2004年浙江省分离株亲缘关系最近。2002～2004年在浙江、福州和2003年山东、江苏省存在引起无菌性脑膜炎的ECHO30的局部流行。
Objective To study the characteristics and molecular epidemiological characteristics of the VP1 gene of aseptic meningitis-Echovirus type 30 (ECHO30) in Fuzhou in 2004. Methods Two strains of ECHO30 (FJ-C2-04, FJ-C7-04) were selected from Fuzhou area in 2004 for RNA extraction and VP1 region reverse transcription-polymerase chain reaction (RT-PCR) The nucleotide sequence of the product. Results The total length of the two ECHO30VP1 genes tested was 876bp, and the translated amino acid was 292aa in length. The homology of VP1 of two viruses was 99.89% (only one bp difference), and the amino acid sequences were identical. The homology of the VP1 region of FJ-C2-04 strain with the related ECHO30 strains retrieved from GenBank was 75.66% -97.49%. The constructed ECHO30 phylogenetic tree divides the known ECHO30 into seven groups, and the ECHO30 isolated in Fuzhou area is in group seven. Conclusion ECHO30 has evolved continuously with the evolution of the era. Fuzhou region isolates and early isolates have the most significant differences, with the closest genetic relationship with Zhejiang isolates in 2004. 2002-2004 in Zhejiang, Fuzhou and 2003 in Shandong Province, Jiangsu Province, causing aseptic meningitis caused by the local epidemic of ECHO30.