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荞麦（Ｆａｇｏｐｙｒｕｍ ｅｓｃｕｌｅｎｔｕｍ Ｍｏｅｎｃｈ．）无菌苗下胚轴切段在含不同激素配比的ＭＳ培养基上进行愈伤组织诱导，发现在 ２． ０ ｍｇ／Ｌ ２． ４－Ｄ与 １． ５ ｍｇ／Ｌ ６ ＢＡ组合时，可１００％地诱导出愈伤组织。愈伤组织在含 １．５ｍｇ／Ｌ ６ＢＡ和１．５ ｍｇ／Ｌ ２．４－Ｄ的激素条件下继代培养１代后转入含２ｍｇ／Ｌ ６ ＢＡ和１ｍｇ／Ｌ ＫＴ的 ＭＳ培养基上，计有８０％以上可分化出苗。转入０．２ｍｇ／Ｌ ＮＡＡ的 １／２ＭＳ培养基上则可诱导出根。研究建立了荞麦离体诱导高频率再生植株的实验体系。
Hypocotyl segments of Fagopyrum esculentum Moench. Were inoculated on MS medium containing different hormone ratios and found in 2. 0 mg / L 2. 4-D and 1. Callus was induced 100% at 5 mg / L 6 BA. Callus was subcultured for 1 generation in hormone containing 1.5 mg / L 6BA and 1.5 mg / L 2.4-D and then transferred to MS medium containing 2 mg / L 6 BA and 1 mg / L KT On, there are more than 80% can be differentiated emergence. Transferred to 0.2mg / L NAA 1 / 2MS medium can be induced root. The experimental system of in vitro induced high-frequency regeneration of buckwheat was established.