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报道用放射配基结合分析法及放射免疫法研究大鼠(小鼠)氢考过多模型α受体数量,亲和力的变化,以及与cGMP的关系。用已建立的α受体测定方法测定了氢考过多模型脑,肾、肝、肺和脾的α1受体最大结合受体数(RT)以及平衡解离常数(KD)。结果表明,脑、肺的RT值无变化,肾和脾分别升高20%、50%,肝则下降10%。KD值均无显著变化。用放免分析法测定了正常和氢考过多模型注射α1激动剂后血浆cGMP的变化,发现氢考组血浆cGMP峰值显著低于正常组。实验表明,糖皮质激素对机体各脏器α1受体数量的影响不一,总的结果导致血浆中cGMP峰值下降,也可能是糖皮质激素直接作用于cGMP系统而引起的结果,脾脏的α1受体可能参与免疫功能的调节。
Reported by radioligand binding assay and radioimmunoassay in rats (mouse) over hydrogen test α receptor number and affinity changes, and cGMP relationship. The maximal receptor alpha (1R) binding receptor (RT) and equilibrium dissociation constant (KD) of α 1 receptor in brain, kidney, liver, lung and spleen were determined by the established α receptor assay. The results showed that the brain and lung RT values ?? did not change, kidney and spleen were increased by 20%, 50%, liver decreased by 10%. KD values did not change significantly. Radioimmunoassay was used to determine the changes of plasma cGMP levels after injection of α1 agonist in normal and hyperimmune models. The plasma cGMP peak value in hydrogen test group was significantly lower than that in normal group. Experiments show that glucocorticoid on the body organ α1 receptors vary in the number of the total result in the plasma cGMP peak decline may also be glucocorticoid direct effect on the cGMP system caused by the results of the spleen α1 The body may be involved in the regulation of immune function.