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以乙型肝炎病毒 (HBV)多聚酶 (P)逆转录酶 (RT)区及表面抗原主蛋白 (HBsAg)序列异质性来探讨HBV准种群状态。用多聚酶链反应 (PCR)方法 ,自 3例慢性HBV患者血清中扩增靶基因 ,克隆入T载体。随机挑选 13株克隆测序 ,发现 2株克隆出现大段缺失 ,另 11株序列之间总差异率为 5 1% ;RT和HBsAg氨基酸序列差异率分别为 4 9%和 7 5 % ;并发现缺失突变、终止突变等多种突变类型。结果提示 ,HBV慢性患者体内有HBV准种群 ,并存在缺陷型HBV病毒
The quasispecies status of HBV was explored by analyzing the heterogeneity of sequence of hepatitis B virus (HBV) polymerase (P) reverse transcriptase (RT) and surface antigen major protein (HBsAg). The target genes were amplified by polymerase chain reaction (PCR) from the serum of 3 patients with chronic HBV and cloned into T vector. Thirteen clones were randomly selected and sequenced. Two clones were found to have a large deletion, with a total percentage difference of 51% in the other 11 sequences. The amino acid sequence differences between RT and HBsAg were 49% and 75%, respectively. Mutations, termination mutations and many other types of mutations. The results suggest that patients with chronic hepatitis B virus quasispecies population, and the presence of defective HBV virus