论文部分内容阅读
目的利用5%葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导建立小鼠肝损伤模型,检测肝组织中鞘氨醇激酶1(Sphingosine kinase-1,Sph K1)的表达情况,探讨其与肝损伤的关系。方法 40只SPF级Balb/C雄性小鼠随机分成4组:对照组和DSS模型组(细分为6周、8周、12周3个小组),每组10只。模型组用5%的DSS溶液按0.6 ml/(d·只)连续灌胃建模,并分别于6周、8周、12周后处死,改良赖氏法检测血清中谷丙转氨酶(ALT)含量,HE染色观察肝脏病理损伤,用于荧光定量PCR检测肝脏Sph K1基因表达水平。结果与对照组比较,各模型组小鼠血清中ALT活性增强,2组比较差异具有统计学意义(P<0.05)。各模型组小鼠肝脏均有明显病理损伤,肉眼观,6周组最为明显;光镜下,各模型组均出现了大片的肝脏坏死,大量淋巴细胞浸润。各模型组的小鼠肝脏组织中Sph K1的表达量下降,12周下降最为明显,与对照比较差异具有统计学意义(P<0.05),Sph K1的表达量与血清中ALT含量基本成反比关系。结论 DSS可诱导小鼠肝损伤模型,肝损伤后小鼠肝内Sph K1也随之表达减少,肝损伤越严重Sph K1表达越少,Sph K1表达与肝脏损伤具有相关性。
Objective To establish a mouse model of liver injury induced by dextran sulfate sodium (DSS) and detect the expression of Sph Kinase 1 (Sph K1) in liver tissue, Injury relationship. Methods Forty SPF Balb / C male mice were randomly divided into 4 groups: control group and DSS model group (subdivided into 3 groups of 6 weeks, 8 weeks and 12 weeks), 10 in each group. The model group was continuously fed with 0.6 ml / (d · d) of 5% DSS solution, and then sacrificed at 6, 8 and 12 weeks respectively. The content of ALT The pathological changes of liver were observed by HE staining and the expression of Sph K1 gene was detected by real-time PCR. Results Compared with the control group, the serum ALT activity of each model group increased, the difference between the two groups was statistically significant (P <0.05). The liver of each model group had obvious pathological lesions, the macroscopic view, the most obvious 6 weeks; under the light microscope, each model group appeared large pieces of liver necrosis, a large number of lymphocyte infiltration. The expression of Sph K1 in the liver of each model group decreased at 12 weeks, and the difference was statistically significant compared with the control (P <0.05). The expression level of Sph K1 was inversely proportional to the level of ALT in serum . Conclusions DSS can induce liver injury in mice. The expression of Sph K1 also decreases in the liver after liver injury. The more the liver injury is, the less the expression of Sph K1 and the expression of Sph K1 are related to liver injury.