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ADP-葡萄糖-焦磷酸化酶(AGPase)是决定植物淀粉生物合成的关键酶,本研究以马铃薯AGP酶基因为候选基因,采用RT-PCR和测序技术检测22个不同类型马铃薯中该基因的遗传变异情况。结果表明,该基因存在多态性,22个品种马铃薯中存在5种类型的酶蛋白,酶蛋白基因型为ISQV时,马铃薯淀粉含量较高,AGP酶蛋白为VSKM和VLKM时,马铃薯淀粉含量较低。
ADP-glucose-pyrophosphorylase (AGPase) is the key enzyme that determines plant starch biosynthesis. In this study, potato AGP enzyme gene was used as a candidate gene, RT-PCR and sequencing techniques were used to detect the genetic inheritance of 22 different types of potato Variation. The results showed that there were polymorphisms in this gene. There were 5 types of enzyme proteins in 22 varieties of potato. When the ISQV genotype was higher, the content of potato starch was higher and AGP protein was VSKM and VLKM, potato starch content was higher low.