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败血症时,白细胞聚集增加,过氧化亚硝酸盐生成也加速,而对于NO合酶敲除的鼠,白细胞聚集增加尤为显著。该研究观察了NO或过氧化亚硝酸盐对白细胞粘附于经内毒素处理的内皮或NO合酶被抑制的内皮的影响。 方法 内皮细胞从新鲜的牛主动脉管腔表面轻轻刮得。白细胞用源自人组织细胞淋巴瘤的类单核细胞系培养,经钙黄绿素荧光染色标记。内
In sepsis, leukocyte accumulation increased and peroxynitrite production also accelerated, whereas leukocyte accumulation was more pronounced in NO synthase knockout mice. This study looked at the effect of NO or peroxynitrite on the adhesion of leukocytes to endothelium that is endotoxin-treated or to which NO synthase is inhibited. Methods Endothelial cells were gently scraped from fresh bovine aortic lumen surface. Leukocytes were cultured with human monocytic lymphoid-derived monocytic cell lines and labeled with calcein fluorochrome. Inside